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Leveraging Human Microbiome Features to Diagnose and Stratify Children with Irritable Bowel Syndrome
- Source :
- The Journal of Molecular Diagnostics : JMD
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Accurate diagnosis and stratification of children with irritable bowel syndrome (IBS) remain challenging. Given the central role of recurrent abdominal pain in IBS, we evaluated the relationships of pediatric IBS and abdominal pain with intestinal microbes and fecal metabolites using a comprehensive clinical characterization and multiomics strategy. Using rigorous clinical phenotyping, we identified preadolescent children (aged 7 to 12 years) with Rome III IBS (n = 23) and healthy controls (n = 22) and characterized their fecal microbial communities using whole-genome shotgun metagenomics and global unbiased fecal metabolomic profiling. Correlation-based approaches and machine learning algorithms identified associations between microbes, metabolites, and abdominal pain. IBS cases differed from controls with respect to key bacterial taxa (eg, Flavonifractor plautii and Lachnospiraceae bacterium 7_1_58FAA), metagenomic functions (eg, carbohydrate metabolism and amino acid metabolism), and higher-order metabolites (eg, secondary bile acids, sterols, and steroid-like compounds). Significant associations between abdominal pain frequency and severity and intestinal microbial features were identified. A random forest classifier built on metagenomic and metabolic markers successfully distinguished IBS cases from controls (area under the curve, 0.93). Leveraging multiple lines of evidence, intestinal microbes, genes/pathways, and metabolites were associated with IBS, and these features were capable of distinguishing children with IBS from healthy children. These multi-omics features, and their links to childhood IBS coupled with nutritional interventions, may lead to new microbiome-guided diagnostic and therapeutic strategies.
- Subjects :
- Male
0301 basic medicine
Abdominal pain
medicine.medical_specialty
Gastroenterology
Statistics, Nonparametric
Article
Pathology and Forensic Medicine
Irritable Bowel Syndrome
Feces
03 medical and health sciences
0302 clinical medicine
Internal medicine
Metabolome
medicine
Humans
Child
Irritable bowel syndrome
2. Zero hunger
Principal Component Analysis
Bacteria
business.industry
Microbiota
Human microbiome
Case-control study
Genomics
medicine.disease
Rome iii
Abdominal Pain
3. Good health
Gastrointestinal Tract
030104 developmental biology
Metagenomics
Case-Control Studies
030220 oncology & carcinogenesis
Multivariate Analysis
Molecular Medicine
Female
medicine.symptom
business
Subjects
Details
- ISSN :
- 15251578
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- The Journal of Molecular Diagnostics
- Accession number :
- edsair.doi.dedup.....2491cac4ebaf3b283d86ad256a176a66