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Colour-encoded lateral flow immunoassay for the simultaneous detection of aflatoxin B1 and type-B fumonisins in a single Test line
- Publication Year :
- 2019
-
Abstract
- A multiplex Lateral Flow Immunoassay was developed based on the use of a single Test line and multicolour gold nanoparticles (GNPs) as signal reporters. Red and blue GNPs were linked to antibodies directed towards two different analytes and included in a typical lateral flow immunoassay configuration, in which the Test line was formed by the mixture of two antigens. As a result of the immunoreactions occurring at the Test zone, diverse combinations of red and blue GNPs labels were captured. Therefore, the Test line assumed different colours depending on which – and how much – analyte is present in the sample. The multiplexing capability of the ‘colour-encoded assay’ is illustrated by the simultaneous detection of aflatoxin B1 (AFB1) and type-B fumonisins (FMs) in wheat and food products that made with wheat. Reproducible detection of AFB1 and FMs contamination in raw and processed food was achieved with visual cut-off levels at 1 ng mL−1 and 50 ng mL−1, respectively. The contaminant was identified based on the colour of the label according with a specific colour code. Furthermore, strips images were acquired by means of a common smartphone and analysed through RGB data analysis providing semi-quantitative detection of the two mycotoxins.
- Subjects :
- Analyte
Aflatoxin
Aflatoxin B1
Blue gold nanoparticles
Immunochromatographic strip test
Multiplex detection
Mycotoxins
RGB data evaluation
Smartphone
Animals
Antibodies
Color
Colorimetry
Edible Grain
Food Contamination
Fumonisins
Gold
Immunoassay
Limit of Detection
Metal Nanoparticles
Rabbits
Triticum
Analytical Chemistry
Chemistry (all)
Biochemistry
Spectroscopy
02 engineering and technology
01 natural sciences
medicine
Multiplex
Detection limit
Chromatography
medicine.diagnostic_test
Chemistry
010401 analytical chemistry
021001 nanoscience & nanotechnology
0104 chemical sciences
Colloidal gold
Line (text file)
0210 nano-technology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....248c77efa6367637445d5b1c8749dec0