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UVB sensitivity and cyclobutane pyrimidine dimer ( CPD) photolyase genotypes in cultivated and wild rice species
- Source :
- Photochemical & Photobiological Sciences. 7:311-320
- Publication Year :
- 2008
- Publisher :
- Springer Science and Business Media LLC, 2008.
-
Abstract
- We investigated the UVB-sensitivity in 12 rice strains belonging to two cultivated species (O. sativa and O. glaberrima) and three wild species (O. barthii, O. meridionalis and O. rufipogon) of rice possessing the AA genome, while focusing on the CPD photolyase activity and the genotypes of CPD photolyase. Although the UVB sensitivity, CPD photolyase activity, and CPD photolyase genotype varied widely among these rice species, the sensitivity to UVB radiation depended on the activity of the CPD photolyase, regardless of grass shape, habitat, or species. The rice strains examined here clearly divided into three groups based on the CPD photolyase activity, and the activity of the strains greatly depended on amino acid residues at positions 126 and 296, with the exception of the W1299 strain (O. meridionalis). The amino acid residues 126 and 296 of CPD photolyase in Sasanishiki strain (O. sativa), which showed higher enzymatic activity and more resistance to UVB, were glutamine (Gln) and Gln, respectively. An amino acid change at position 126 from Gln to arginine ("Nori"-type) in the photolyase led to a reduction of enzymatic activity. Additionally, an amino acid change at position 296 from Gln to histidine led to a further reduction in activity. The activity of the W1299 strain, which possesses a "Nori"-type CPD photolyase, was the highest among the strains examined here, and was similar to that of the Sasanishiki. The CPD photolyase of the W1299 contains ten amino acid substitutions, compared to Sasanishiki. The alterations in amino acid residues in the W1299 CPD photolyase compensated for the reduction in activity caused by the amino acid substitutions at positions 126. Knowledge of the activity of different CPD photolyase genotypes will be useful in developing improved rice cultivars.
- Subjects :
- Genotype
Arginine
Ultraviolet Rays
Molecular Sequence Data
education
Pyrimidine dimer
Biology
Species Specificity
Point Mutation
Amino Acid Sequence
Physical and Theoretical Chemistry
Photolyase
Histidine
Plant Proteins
chemistry.chemical_classification
Sequence Homology, Amino Acid
Strain (chemistry)
food and beverages
Dose-Response Relationship, Radiation
Oryza
Amino acid
Enzyme Activation
Glutamine
Enzyme
Biochemistry
chemistry
Pyrimidine Dimers
Deoxyribodipyrimidine Photo-Lyase
DNA Damage
Subjects
Details
- ISSN :
- 14749092 and 1474905X
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Photochemical & Photobiological Sciences
- Accession number :
- edsair.doi.dedup.....2486cb8ae4095c2bba254029bb9f911a