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Lagooning of wastewaters favors dissemination of clinically relevant Pseudomonas aeruginosa
- Source :
- Research in Microbiology, Research in Microbiology, Elsevier, 2013, 164 (8), pp.856-66. ⟨10.1016/j.resmic.2013.06.007⟩, Research in Microbiology, Elsevier, 2013, 164 (8), pp.856-66. 〈10.1016/j.resmic.2013.06.007〉
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- International audience; The significance of wastewater treatment lagoons (WWTLs) as point sources of clinically relevant Pseudomonas aeruginosa that can disseminate through rural and peri-urban catchments was investigated. A panel of P. aeruginosa strains collected over three years from WWTLs and community-acquired infections was compared by pulsed field gel electrophoresis (PFGE) DNA fingerprinting and multilocus sequence typing (MLST). Forty-four distantly related PFGE profiles and four clonal complexes were found among the WWTL strains analyzed. Some genotypes were repeatedly detected from different parts of WWTLs, including the influent, suggesting an ability to migrate and persist over time. MLST showed all investigated lineages to match sequence types described in other countries and strains from major clinical clones such as PA14 of ST253 and "C" of ST17 were observed. Some of these genotypes matched isolates from community-acquired infections recorded in the WWTL geographic area. Most WWTL strains harbored the main P. aeruginosa virulence genes; 13% harbored exoU-encoded cytoxins, but on at least six different genomic islands, with some of these showing signs of genomic instability. P. aeruginosa appeared to be highly successful opportunistic colonizers of WWTLs. Lagooning of wastewaters was found to favor dissemination of clinically relevant P. aeruginosa among peri-urban watersheds.
- Subjects :
- Genotype
Virulence Factors
Virulence
Biology
Wastewater
medicine.disease_cause
Microbiology
03 medical and health sciences
[ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology
medicine
Pulsed-field gel electrophoresis
Pseudomonas Infections
Molecular Biology
Gene
030304 developmental biology
0303 health sciences
030306 microbiology
Pseudomonas aeruginosa
General Medicine
Sequence types
6. Clean water
Electrophoresis, Gel, Pulsed-Field
Community-Acquired Infections
Molecular Typing
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
DNA profiling
Multilocus sequence typing
Subjects
Details
- Language :
- English
- ISSN :
- 09232508
- Database :
- OpenAIRE
- Journal :
- Research in Microbiology, Research in Microbiology, Elsevier, 2013, 164 (8), pp.856-66. ⟨10.1016/j.resmic.2013.06.007⟩, Research in Microbiology, Elsevier, 2013, 164 (8), pp.856-66. 〈10.1016/j.resmic.2013.06.007〉
- Accession number :
- edsair.doi.dedup.....247e2a1f066fdeda23fa90630bb82049