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Transcriptional profiling of human mesenchymal stem cells transduced with reporter genes for imaging
- Source :
- Physiological Genomics. 37:23-34
- Publication Year :
- 2009
- Publisher :
- American Physiological Society, 2009.
-
Abstract
- Mesenchymal stem cells (MSCs) can differentiate into osteogenic, adipogenic, chondrogenic, myocardial, or neural lineages when exposed to specific stimuli, making them attractive for tissue repair and regeneration. We have used reporter gene-based imaging technology to track MSC transplantation or implantation in vivo. However, the effects of lentiviral transduction with the fluc-mrfp-ttk triple-fusion vector on the transcriptional profiles of MSCs remain unknown. In this study, gene expression differences between wild-type and transduced hMSCs were evaluated using an oligonucleotide human microarray. Significance Analysis of Microarray identified differential genes with high accuracy; RT-PCR validated the microarray results. Annotation analysis showed that transduced hMSCs upregulated cell differentiation and antiapoptosis genes while downregulating cell cycle, proliferation genes. Despite transcriptional changes associated with bone and cartilage remodeling, their random pattern indicates no systematic change of crucial genes that are associated with osteogenic, adipogenic, or chondrogenic differentiation. This correlates with the experimental results that lentiviral transduction did not cause the transduced MSCs to lose their basic stem cell identity as demonstrated by osteogenic, chondrogenic, and adipogenic differentiation assays with both transduced and wild-type MSCs, although a certain degree of alterations occurred. Histological analysis demonstrated osteogenic differentiation in MSC-loaded ceramic cubes in vivo. In conclusion, transduction of reporter genes into MSCs preserved the basic properties of stem cells while enabling noninvasive imaging in living animals to study the biodistribution and other biological activities of the cells.
- Subjects :
- Pluripotent Stem Cells
Ceramics
Transcription, Genetic
Physiology
Cellular differentiation
Biology
Mesenchymal Stem Cell Transplantation
Thymidine Kinase
Prosthesis Implantation
Mice
Genes, Reporter
Transduction, Genetic
Genetics
Animals
Humans
Gene Regulatory Networks
Whole Body Imaging
Luciferases
Induced pluripotent stem cell
Oligonucleotide Array Sequence Analysis
Reporter gene
Adipogenesis
Gene Expression Profiling
Mesenchymal stem cell
Mesenchymal Stem Cells
Cell cycle
Molecular biology
Cell biology
Gene expression profiling
Transplantation
Luminescent Proteins
Biological Assay
Stem cell
Software
Research Article
Subjects
Details
- ISSN :
- 15312267 and 10948341
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Physiological Genomics
- Accession number :
- edsair.doi.dedup.....247dae435804bef252f32f11fced0bb5
- Full Text :
- https://doi.org/10.1152/physiolgenomics.00300.2007