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Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples
- Source :
- Analytical and Bioanalytical Chemistry
- Publication Year :
- 2020
- Publisher :
- Springer Berlin Heidelberg, 2020.
-
Abstract
- Trace elements are essential for life and their concentration in cells and tissues must be tightly maintained and controlled to avoid pathological conditions. Established methods to measure the concentration of trace elements in biological matrices often provide only single element information, are time-consuming, and require special sample preparation. Therefore, the development of straightforward and rapid analytical methods for enhanced, multi-trace element determination in biological samples is an important and raising field of trace element analysis. Herein, we report on the development and validation of a reliable method based on total reflection X-ray fluorescence (TXRF) analysis to precisely quantify iron and other trace metals in a variety of biological samples, such as the liver, parenchymal and non-parenchymal liver cells, and bone marrow–derived macrophages. We show that TXRF allows fast and simple one-point calibration by addition of an internal standard and has the potential of multi-element analysis in minute sample amounts. The method was validated for iron by recovery experiments in homogenates in a wide concentration range from 1 to 1600 μg/L applying well-established graphite furnace atomic absorption spectrometry (GFAAS) as a reference method. The recovery rate of 99.93 ± 0.14% reveals the absence of systematic errors. Furthermore, the standard reference material “bovine liver” (SRM 1577c, NIST) was investigated in order to validate the method for further biometals. Quantitative recoveries (92–106%) of copper, iron, zinc, and manganese prove the suitability of the developed method. The limits of detection for the minute sample amounts are in the low picogram range.<br />publishedVersion
- Subjects :
- DDC 540 / Chemistry & allied sciences
Liver tissues
Manganese
01 natural sciences
Biochemistry
Liver cells
Analytical Chemistry
Mice
DDC 570 / Life sciences
HUMAN HAIR
Limit of Detection
Sample preparation
TXRF ANALYSIS
Cells, Cultured
0303 health sciences
Totalreflexionsröntgenfluoreszenzanalyse
HEALTHY TISSUES
Verdauung
Liver
Metals
ddc:540
Digestion
Total reflection X-ray fluorescence spectrometry
Research Paper
QUANTITATION
Materials science
Iron
chemistry.chemical_element
X-ray fluorescence
Zinc
Mass spectrometry
03 medical and health sciences
ddc:570
WHOLE-BLOOD
Calibration
Animals
Bone marrow-derived macrophages
Iron trace analysis
EXPOSURE
Bone marrow–derived macrophages
030304 developmental biology
Detection limit
Chromatography
Macrophages
010401 analytical chemistry
Leberepithelzelle
Spectrometry, X-Ray Emission
0104 chemical sciences
Trace Elements
chemistry
Biometal trace analysis
MULTIELEMENT DETERMINATION
Cattle
Graphite furnace atomic absorption
Subjects
Details
- Language :
- English
- ISSN :
- 16182650 and 16182642
- Volume :
- 412
- Issue :
- 24
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....24736d8221f240e3572f6bb0183fc586