Back to Search
Start Over
CEACAM5 overexpression is a reliable characteristic of CD133-positive colorectal cancer stem cells
- Source :
- Cancer biomarkers : section A of Disease markers. 32(1)
- Publication Year :
- 2021
-
Abstract
- BACKGROUND: CD133 (prominin-1) is the most commonly used molecular marker of the cancer stem cells (CSCs) that maintain tumor progression and recurrence in colorectal cancer. However, the proteome of CSCs directly isolated from colorectal tumors based on CD133 expression has never been investigated. OBJECTIVE: To reveal biomarkers of CD133-positive colorectal CSCs. METHODS: Thirty colorectal tumor samples were collected from patients undergoing bowel resection. CD133-positive and CD133-negative cells were isolated by FACS. Comparative proteomic profiling was performed by LC-MS/MS analysis combined with label-free quantification. Verification of differentially expressed proteins was performed by flow cytometry or ELISA. CD133-knockout Caco-2 and HT-29 cell lines were generated using CRISPR-Cas9 gene editing. RESULTS: LC-MS/MS analysis identified 29 proteins with at least 2.5-fold higher expression in CD133-positive cells versus CD133-negative cells. Flow cytometry confirmed CEACAM5 overexpression in CD133-positive cells in all clinical samples analyzed. S100A8, S100A9, and DEFA1 were differentially expressed in only a proportion of the samples. CD133 knockout in the colon cancer cell lines Caco-2 and HT-29 did not affect the median level of CEACAM5 expression, but led to higher variance of the percentage of CEACAM5-positive cells. CONCLUSIONS: High CEACAM5 expression in colorectal cancer cells is firmly associated with the CD133-positive colorectal CSC phenotype, but it is unlikely that CD133 directly regulates CEACAM5 expression.
- Subjects :
- Cancer Research
Colorectal cancer
Biology
GPI-Linked Proteins
S100A9
Flow cytometry
03 medical and health sciences
0302 clinical medicine
Cancer stem cell
Genetics
medicine
Humans
AC133 Antigen
neoplasms
030304 developmental biology
0303 health sciences
medicine.diagnostic_test
General Medicine
medicine.disease
Carcinoembryonic Antigen
carbohydrates (lipids)
Oncology
Tumor progression
Cell culture
030220 oncology & carcinogenesis
embryonic structures
Proteome
Colonic Neoplasms
Cancer research
Neoplastic Stem Cells
Stem cell
Subjects
Details
- ISSN :
- 18758592
- Volume :
- 32
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cancer biomarkers : section A of Disease markers
- Accession number :
- edsair.doi.dedup.....2469f1fb2e49d2e0041361ec8fcfb0ec