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Human adipose-derived stromal/stem cells expressing doublecortin improve cartilage repair in rabbits and monkeys
- Source :
- npj Regenerative Medicine, Vol 6, Iss 1, Pp 1-16 (2021), NPJ Regenerative Medicine
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- Localized cartilage lesions in early osteoarthritis and acute joint injuries are usually treated surgically to restore function and relieve pain. However, a persistent clinical challenge remains in how to repair the cartilage lesions. We expressed doublecortin (DCX) in human adipose-derived stromal/stem cells (hASCs) and engineered hASCs into cartilage tissues using an in vitro 96-well pellet culture system. The cartilage tissue constructs with and without DCX expression were implanted in the knee cartilage defects of rabbits (n = 42) and monkeys (n = 12). Cohorts of animals were euthanized at 6, 12, and 24 months after surgery to evaluate the cartilage repair outcomes. We found that DCX expression in hASCs increased expression of growth differentiation factor 5 (GDF5) and matrilin 2 in the engineered cartilage tissues. The cartilage tissues with DCX expression significantly enhanced cartilage repair as assessed macroscopically and histologically at 6, 12, and 24 months after implantation in the rabbits and 24 months after implantation in the monkeys, compared to the cartilage tissues without DCX expression. These findings suggest that hASCs expressing DCX may be engineered into cartilage tissues that can be used to treat localized cartilage lesions.
- Subjects :
- Pathology
medicine.medical_specialty
Stromal cell
biology
business.industry
Cartilage
Biomedical Engineering
Medicine (miscellaneous)
Growth differentiation factor
Adipose tissue
Cell Biology
GDF5
Article
In vitro
Doublecortin
medicine.anatomical_structure
Regenerative medicine
medicine
biology.protein
Mesenchymal stem cells
Medicine
Stem cell
business
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 20573995
- Volume :
- 6
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- npj Regenerative Medicine
- Accession number :
- edsair.doi.dedup.....2462ee82b4055ee9b284773b2581e144