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Effects of methyl gallate and gallic acid on the production of inflammatory mediators interleukin-6 and interleukin-8 by oral epithelial cells stimulated with Fusobacterium nucleatum
- Source :
- The Journal of Microbiology. 47:760-767
- Publication Year :
- 2009
- Publisher :
- Springer Science and Business Media LLC, 2009.
-
Abstract
- Interactions between periodontal bacteria and human oral epithelial cells can lead to the activation and expression of a variety of inflammatory mediators in epithelial cells. Fusobacterium nucleatum is a filamentous human pathogen that is strongly associated with periodontal diseases. This study examined the effects of methyl gallate (MG) and gallic acid (GA) on the production of inflammatory mediators, interleukin (IL)-6 and IL-8, by oral epithelial cells stimulated by F. nucleatum. In a real-time reverse transcription-polymerase chain reaction and an enzyme-linked immunosorbent assay, live F. nucleatum induced high levels of gene expression and protein release of IL-6 and IL-8. The effects of MG and GA were examined by treating KB oral epithelial cells with MG and GA and stimulating them with F. nucleatum. MG and GA inhibited significantly the increases in the IL-6 and IL-8 gene and protein levels in a dose-dependent manner. These Compounds also inhibited the growth of F. nucleatum. No visible effects of MG and GA on the adhesion and invasion of KB cells by F. nucleatum were observed. In conclusion, both MG and GA inhibit IL-6 and IL-8 production from F. nucleatum-activated KB cells.
- Subjects :
- Enzyme-Linked Immunosorbent Assay
Biology
Applied Microbiology and Biotechnology
Microbiology
Cell Line
chemistry.chemical_compound
stomatognathic system
Gallic Acid
Gene expression
Humans
Immunologic Factors
Gallic acid
Interleukin 8
Methyl gallate
Interleukin 6
Fusobacterium nucleatum
Interleukin-6
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Profiling
Interleukin-8
Interleukin
Epithelial Cells
General Medicine
biology.organism_classification
Molecular biology
stomatognathic diseases
chemistry
Cell culture
biology.protein
Subjects
Details
- ISSN :
- 19763794 and 12258873
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- The Journal of Microbiology
- Accession number :
- edsair.doi.dedup.....24619f13da2416e256292b410dcca7f8
- Full Text :
- https://doi.org/10.1007/s12275-009-0097-7