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Effects of methyl gallate and gallic acid on the production of inflammatory mediators interleukin-6 and interleukin-8 by oral epithelial cells stimulated with Fusobacterium nucleatum

Authors :
Jong-Suk Oh
Hoi-Soon Lim
Hee-Sook Jang
Kyu-Ho Yang
Mi-Sun Kang
Nam-Ki Choi
Seon-Mi Kim
Source :
The Journal of Microbiology. 47:760-767
Publication Year :
2009
Publisher :
Springer Science and Business Media LLC, 2009.

Abstract

Interactions between periodontal bacteria and human oral epithelial cells can lead to the activation and expression of a variety of inflammatory mediators in epithelial cells. Fusobacterium nucleatum is a filamentous human pathogen that is strongly associated with periodontal diseases. This study examined the effects of methyl gallate (MG) and gallic acid (GA) on the production of inflammatory mediators, interleukin (IL)-6 and IL-8, by oral epithelial cells stimulated by F. nucleatum. In a real-time reverse transcription-polymerase chain reaction and an enzyme-linked immunosorbent assay, live F. nucleatum induced high levels of gene expression and protein release of IL-6 and IL-8. The effects of MG and GA were examined by treating KB oral epithelial cells with MG and GA and stimulating them with F. nucleatum. MG and GA inhibited significantly the increases in the IL-6 and IL-8 gene and protein levels in a dose-dependent manner. These Compounds also inhibited the growth of F. nucleatum. No visible effects of MG and GA on the adhesion and invasion of KB cells by F. nucleatum were observed. In conclusion, both MG and GA inhibit IL-6 and IL-8 production from F. nucleatum-activated KB cells.

Details

ISSN :
19763794 and 12258873
Volume :
47
Database :
OpenAIRE
Journal :
The Journal of Microbiology
Accession number :
edsair.doi.dedup.....24619f13da2416e256292b410dcca7f8
Full Text :
https://doi.org/10.1007/s12275-009-0097-7