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H3K27me3 profiling of the endosperm implies exclusion of polycomb group protein targeting by DNA methylation
- Source :
- PLoS Genetics, Vol 6, Iss 10 (2010), PLoS Genetics, 6 (10). e1001152., PLoS genetics, PLoS Genetics, PLoS Genetics, 6 (10)
- Publication Year :
- 2010
- Publisher :
- Public Library of Science (PLoS), 2010.
-
Abstract
- Polycomb group (PcG) proteins act as evolutionary conserved epigenetic mediators of cell identity because they repress transcriptional programs that are not required at particular developmental stages. Each tissue is likely to have a specific epigenetic profile, which acts as a blueprint for its developmental fate. A hallmark for Polycomb Repressive Complex 2 (PRC2) activity is trimethylated lysine 27 on histone H3 (H3K27me3). In plants, there are distinct PRC2 complexes for vegetative and reproductive development, and it was unknown so far whether these complexes have target gene specificity. The FERTILIZATION INDEPENDENT SEED (FIS) PRC2 complex is specifically expressed in the endosperm and is required for its development; loss of FIS function causes endosperm hyperproliferation and seed abortion. The endosperm nourishes the embryo, similar to the physiological function of the placenta in mammals. We established the endosperm H3K27me3 profile and identified specific target genes of the FIS complex with functional roles in endosperm cellularization and chromatin architecture, implicating that distinct PRC2 complexes have a subset of specific target genes. Importantly, our study revealed that selected transposable elements and protein coding genes are specifically targeted by the FIS PcG complex in the endosperm, whereas these elements and genes are densely marked by DNA methylation in vegetative tissues, suggesting that DNA methylation prevents targeting by PcG proteins in vegetative tissues.<br />PLoS Genetics, 6 (10)<br />ISSN:1553-7390<br />ISSN:1553-7404
- Subjects :
- 0106 biological sciences
Cancer Research
Arabidopsis
Polycomb-Group Proteins
01 natural sciences
Genetics and Genomics/Plant Genetics and Gene Expression
Endosperm
Histones
Gene Expression Regulation, Plant
Cluster Analysis
Genetics (clinical)
Molecular Biology/DNA Methylation
Oligonucleotide Array Sequence Analysis
Regulation of gene expression
Genetics
0303 health sciences
biology
Reverse Transcriptase Polymerase Chain Reaction
digestive, oral, and skin physiology
food and beverages
Flow Cytometry
Plants, Genetically Modified
Chromatin
DNA methylation
PRC2
Genome, Plant
Research Article
lcsh:QH426-470
Green Fluorescent Proteins
macromolecular substances
Molecular Biology/Histone Modification
Methylation
03 medical and health sciences
Genetics and Genomics/Epigenetics
Polycomb-group proteins
Epigenetics
Genetics and Genomics/Genomics
Molecular Biology/Chromatin Structure
Molecular Biology
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
Arabidopsis Proteins
Gene Expression Profiling
Lysine
fungi
Endosperm cellularization
DNA Methylation
Repressor Proteins
lcsh:Genetics
Microscopy, Fluorescence
Developmental Biology/Plant Growth and Development
Mutation
biology.protein
DNA Transposable Elements
010606 plant biology & botany
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 15537404 and 15537390
- Volume :
- 6
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- PLoS Genetics
- Accession number :
- edsair.doi.dedup.....245adcf045ff4d9a7210dbb5f97080ae