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Tubular dentin formation by TGF‐β/BMP signaling in dental epithelial cells
- Source :
- Oral Diseases. 29:1644-1656
- Publication Year :
- 2022
- Publisher :
- Wiley, 2022.
-
Abstract
- This study aimed to identify formation of tubular dentin induced by transforming growth factor-β (TGF-β) and bone morphogenic protein (BMP) signaling pathway in dental epithelial cells.We collected conditioned medium (CM) of rTGF-β1/rBMP-2-treated HAT-7 and treated to MDPC-23 cells. The expression levels of odontoblast differentiation markers, KLF4, DMP1, and DSP were evaluated by real-time PCR and Western blot analysis. To evaluate whether CM of rTGF-β1/rBMP-2 induces tubular dentin formation, we made a beagle dog tooth defect model.Here, we show that Cpne7 is regulated by Smad4-dependent TGF-β1/BMP2 signaling pathway in dental epithelial cells. CM of rTGF-β1/rBMP-2 treated HAT-7 or rCPNE7 raises the expression levels of KLF4, DMP1, and DSP in MDPC-23 cells. When rTGF-β1 or rBMP-2 is directly treated to MDPC-23 cells, however, expression levels of Cpne7-regulated genes remain unchanged. In a beagle dog defect model, application of rTGF-β1/BMP2-treated CM resulted in tubular tertiary dentin mixed with osteodentin at cavity-prepared sites, while rTGF-β1 group exhibited homogenous osteodentin.Taken together, Smad4-dependent TGF-β1/BMP2 signaling regulates Cpne7 in dental epithelial cells, and CPNE7 protein secreted from pre-ameloblasts mediates odontoblast differentiation via epithelial-mesenchymal interaction.
- Subjects :
- Otorhinolaryngology
General Dentistry
Subjects
Details
- ISSN :
- 16010825 and 1354523X
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- Oral Diseases
- Accession number :
- edsair.doi.dedup.....2446fe0891f5b8c028fa88b0de38e42d