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Identification and Characterization ofEurotium rubrumIsolated from Meju in Korea
- Source :
- Mycobiology
- Publication Year :
- 2009
- Publisher :
- Mycobiology (KAMJE), 2009.
-
Abstract
- We isolated and identified a strain of Eurotium rubrum from Meju that has not been reported in Korea. This fungus is yellowish brown; reverse dark brown on CYA and PDA while yellow on 2% MEA at 25℃. Cleistothecia are first bright yellow and gradually turned brown. Mycerial growth on CYA attained a diameter of 30 mm at 20℃, 37 mm at 25℃ and 32 mm at 30℃ after 15 days. The isolate grew slower on 2% MEA (< 20 mm 15 days at 25℃) compared to CYA and PDA (< 40 mm 15 days at 25℃). Cleistothecia are superficial, yellow to light brown, globose to subglobose, 40~75 µm in diameter. Asci are 8-spored and globose to subglobose 8~11 µm. Ascospores are disciform, 4.0~5.0 µm in length and 4.2~4.5 µm in width. Conidia are ovate or bacillar, finely roughened to densely spinulose, 4.6~6.0 µm in length and 3.0~4.3 µm in width. Compared to known Eurotium rubrum, the Korean isolate showed 99% sequence similarity in ITS rDNA (554 bp) and calmodulin (750 bp) gene and 100% in β-tubulin (1016 bp) gene. The E. rubrum isolate also had weak β-glucosidase and protease activities.
Details
- ISSN :
- 12298093
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Mycobiology
- Accession number :
- edsair.doi.dedup.....242ead4de98647a10dfc619326f8bb0d