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Effect of chronic tetrahydrobiopterin supplementation on blood pressure and proteinuria in 5/6 nephrectomized rats
- Source :
- Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association. 19(9)
- Publication Year :
- 2004
-
Abstract
- Background. Tetrahydrobiopterin (BH4) is a key cofactor of nitric oxide (NO) synthase. Reduced BH4 levels may mediate endothelial NO synthase uncoupling, resulting in reduced NO synthesis and enhanced oxidative stress. In rats after 5/6 nephrectomy (Nx), administration of BH4 prevents the onset of hypertension, typically observed 10 days after Nx. This effect is associated with an increased synthesis of NO. The aim of the present study was to evaluate the effect of chronic BH4 therapy on blood pressure and renal morphology. Methods. During an 8 week period, five groups of rats were studied: untreated 5/6 Nx rats, BH4-treated Nx rats (BH4, 10 mg/kg body weight/day administered intraperitoneally), L-arginine treated Nx rats (LA, 130 mg/kg/day), diltiazem-treated Nx rats (DILT, 30 mg/kg/day) and sham-operated rats. Treatments were commenced 24 h after surgery. Systolic blood pressure values (SBP), 24 h proteinuria (UP) and creatinine clearance rate (CCR) were assessed before and at weeks 4 and 8 of the study period. Histological changes in the kidney were evaluated at the end of the study (week 8). Results. Compared with baseline, in Nx rats both SBP and UP increased significantly (112±1 to 136± 1.4 mmHg, P
- Subjects :
- Male
medicine.medical_specialty
medicine.medical_treatment
Vasodilator Agents
Renal function
Blood Pressure
Arginine
Nitric Oxide
Nephrectomy
Antioxidants
Diltiazem
Internal medicine
medicine
Animals
Rats, Wistar
Antihypertensive Agents
Transplantation
Kidney
Proteinuria
biology
business.industry
medicine.disease
Biopterin
Glomerular Mesangium
Rats
Nitric oxide synthase
Oxidative Stress
Endocrinology
Blood pressure
medicine.anatomical_structure
Nephrology
Hypertension
Models, Animal
biology.protein
Kidney Failure, Chronic
medicine.symptom
business
Kidney disease
medicine.drug
Subjects
Details
- ISSN :
- 09310509
- Volume :
- 19
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
- Accession number :
- edsair.doi.dedup.....2428f0bf227f227be5a3e3ade15b0f25