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Noninvasive population genetics: a review of sample source, diet, fragment length and microsatellite motif effects on amplification success and genotyping error rates
- Source :
- Conservation Genetics, vol. 8, no. 1, pp. 249-260, Conservation Genetics, Conservation Genetics, Springer Verlag, 2007, 8 (1), pp.249-260, Conservation Genetics, 2007, 8 (1), pp.249-260
- Publication Year :
- 2007
- Publisher :
- HAL CCSD, 2007.
-
Abstract
- International audience; Noninvasive population genetics has found many applications in ecology and conservation biology. However, the technical difficulties inherent to the analysis of low quantities of DNA generally tend to limit the efficiency of this approach. The nature of samples and loci used in noninvasive population genetics are important factors that may help increasing the potential success of case studies. Here we reviewed the effects of the source of DNA (hair vs. faeces), the diet of focal species, the length of mitochondrial DNA fragments, and the length and repeat motif of nuclear microsatellite loci on genotyping success (amplification success and rate of allelic dropout). Locus-specific effects appeared to have the greatest impact, amplification success decreasing with both mitochondrial and microsatellite fragments' length, while error rates increase with amplicons' length. Dinucleotides showed best amplification success and lower error rates compared to longer repeat units. Genotyping success did not differ between hair- versus faeces-extracted DNA, and success in faeces-based analyses was not consistently influenced by the diet of focal species. While the great remaining variability among studies implies that other unidentified parameters are acting, results show that the careful choice of genetic markers may allow optimizing the success of noninvasive approaches.
- Subjects :
- 0106 biological sciences
Mitochondrial DNA
amplification success
Population genetics
Biology
010603 evolutionary biology
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
noninvasive
Genetics
allelic dropout
Allele
Genotyping
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
0303 health sciences
Amplicon
genotyping errors
[SDE.BE] Environmental Sciences/Biodiversity and Ecology
chemistry
Genetic marker
Microsatellite
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
low DNA
DNA
Subjects
Details
- Language :
- English
- ISSN :
- 15660621 and 15729737
- Database :
- OpenAIRE
- Journal :
- Conservation Genetics, vol. 8, no. 1, pp. 249-260, Conservation Genetics, Conservation Genetics, Springer Verlag, 2007, 8 (1), pp.249-260, Conservation Genetics, 2007, 8 (1), pp.249-260
- Accession number :
- edsair.doi.dedup.....241e8bd7ab18d8ce37418f296998092e