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In vivo molecular imaging of myocardial angiogenesis using the αvβ3 integrin-targeted tracer 99mTc-RAFT-RGD
- Source :
- Journal of Nuclear Cardiology, Journal of Nuclear Cardiology, Springer Verlag, 2010, 17 (3), pp.435-443. ⟨10.1007/s12350-010-9191-9⟩
- Publication Year :
- 2010
- Publisher :
- HAL CCSD, 2010.
-
Abstract
- Myocardial angiogenesis following reperfusion of an infarcted area may impact on patient prognosis and pro-angiogenic treatments are currently evaluated. The non-invasive imaging of angiogenesis would therefore be of potential clinical relevance in these settings. (99m)Tc-RAFT-RGD is a novel (99m)Tc-labeled tracer that targets the alpha(v)beta(3) integrin. Our objective was to determine whether this tracer was suitable for myocardial angiogenesis imaging.A rat model of reperfused myocardial infarction was employed. Fourteen days following reperfusion, the animals were injected with (99m)Tc-RAFT-RGD or with its negative control (99m)Tc-RAFT-RAD. Fourteen animals were dedicated to autoradiographic imaging, infarct staining, and gamma-well counting of myocardial activity. In vivo dual-isotope pinhole SPECT imaging of (201)Tl and (99m)Tc-RAFT-RGD or (99m)Tc-RAFT-RAD was also performed in 11 additional animals. Neovessels were observed by immunostaining in the infarcted and peri-infarct areas. (99m)Tc-RAFT-RGD infarct-to-normal ratios by gamma-well counting and ex vivo imaging (2.5 +/- 0.6 and 4.9 +/- 0.9, respectively) were significantly higher than those of (99m)Tc-RAFT-RAD (1.7 +/- 0.2 and 2.2 +/- 0.4, respectively, P.05). The infarcted area was readily visible in vivo by SPECT with (99m)Tc-RAFT-RGD but not with (99m)Tc-RAFT-RAD (infarct-to-normal zone activity ratio, 2.5 +/- 0.6 and 1.7 +/- 0.4, respectively, P.05).(99m)Tc-RAFT-RGD allowed the experimental in vivo molecular imaging of myocardial angiogenesis.
- Subjects :
- Male
Pathology
medicine.medical_specialty
Biodistribution
Angiogenesis
Neovascularization, Physiologic
Myocardial Reperfusion Injury
030204 cardiovascular system & hematology
Peptides, Cyclic
Iodine Radioisotopes
03 medical and health sciences
0302 clinical medicine
In vivo
Spect imaging
medicine
Animals
[CHIM]Chemical Sciences
Tissue Distribution
Radiology, Nuclear Medicine and imaging
Rats, Wistar
Radionuclide Imaging
Cells, Cultured
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
0303 health sciences
αvβ3 integrin
business.industry
Organotechnetium Compounds
Integrin alphaVbeta3
Immunohistochemistry
Rats
3. Good health
Autoradiography
Radiopharmaceuticals
Molecular imaging
Cardiology and Cardiovascular Medicine
business
Immunostaining
Ex vivo
Subjects
Details
- Language :
- English
- ISSN :
- 10713581
- Database :
- OpenAIRE
- Journal :
- Journal of Nuclear Cardiology, Journal of Nuclear Cardiology, Springer Verlag, 2010, 17 (3), pp.435-443. ⟨10.1007/s12350-010-9191-9⟩
- Accession number :
- edsair.doi.dedup.....24112487b64a46c0c7641622a4462bbd