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TLR4 promotes the expression of HIF-1α by triggering reactive oxygen species in cervical cancer cells in vitro-implications for therapeutic intervention
- Source :
- Molecular Medicine Reports
- Publication Year :
- 2017
-
Abstract
- The present study investigated the mechanism underlying Toll-like receptor 4 (TLR4)-mediated stimulation of hypoxia-inducible factor-1α (HIF-1α) activity and its association with reactive oxygen species (ROS) in cervical cancer cells. SiHa cells were cultured and randomized to control, lipopolysaccharide (LPS), methyl-β-cyclodextrin (MβCD)+LPS, ammonium pyrrolidinedithiocarbamate (PDTC)+LPS, ST2825+LPS and small interfering (si) RNA TLR4+LPS treatment groups. Cell proliferation was quantified using an MTT assay, cell cloning was performed using soft agar colony formation and HIF-1α expression was detected by immunocytochemical staining and western blot analyses. Dichloro-dihydro-fluorescein diacetate and lucigenin luminescence assays were used to detect alterations in ROS and nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase content, respectively. Co-localization of TLR4 and HIF-1α was detected by immunofluorescence staining and observed using fluorescence microscopy. Compared with the control group, cell proliferation was enhanced in the LPS-treated group and was not altered in the PDTC+LPS treatment group. Cell proliferation was reduced in all other treatment groups (P
- Subjects :
- 0301 basic medicine
Cancer Research
Lipopolysaccharide
cervical cancer
Uterine Cervical Neoplasms
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Cell Line, Tumor
Genetics
Humans
MTT assay
Molecular Biology
Tumor Stem Cell Assay
Cell Proliferation
chemistry.chemical_classification
Oxidase test
Reactive oxygen species
NADPH oxidase
biology
hypoxia-inducible factor-1α
Cell growth
NADPH Oxidases
Articles
Hypoxia-Inducible Factor 1, alpha Subunit
Toll-like receptor 4
Molecular biology
lipid raft
Gene Expression Regulation, Neoplastic
Protein Transport
030104 developmental biology
Oncology
chemistry
Apoptosis
030220 oncology & carcinogenesis
Immunology
TLR4
biology.protein
Molecular Medicine
lipids (amino acids, peptides, and proteins)
Female
Reactive Oxygen Species
Protein Binding
Subjects
Details
- ISSN :
- 17913004
- Volume :
- 17
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Molecular medicine reports
- Accession number :
- edsair.doi.dedup.....2405e63a47db2e3c93ae2c75dc92a10e