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Pregabalin Ameliorates Lipopolysaccharide-Induced Pancreatic Inflammation in Aged Rats
- Source :
- Endocrine, Metabolic & Immune Disorders - Drug Targets. 19:1141-1147
- Publication Year :
- 2019
- Publisher :
- Bentham Science Publishers Ltd., 2019.
-
Abstract
- Objective: The aim of this study was to examine pancreatic pathology and the prophylactic effects of pregabalin in lipopolysaccharide (LPS) induced sepsis model in aged rats. Methods: Twenty-four female, one-year-old, Wistar Albino rats were assigned to three groups; Group I (control), Group II (study group: 5mg/kg LPS intraperitoneal, single dose) and Group III(treatment group: 5mg/kg LPS+30 mg/kg oral pregabalin one hour before LPS). Animals were sacrificed by exsanguination 6 hours after LPS administration. Blood and pancreatic tissue samples were collected for biochemical, pathological, and immunohistochemical analyses. Results: LPS caused increases in serum amylase and lipase level but led to a reduction in glucose levels. Following histopathological analysis, numerous neutrophil leucocyte infiltrations were observed in vessels and pancreatic tissues. Increased caspase-3 expression was observed in both the endocrine and exocrine pancreas in the LPS group. Similarly, IL-6, caspase-3 (Cas-3), inducible nitric oxide synthase (iNOS), granulocyte colony-stimulating factor (G-CSF) and serum amyloid-A (SAA) expressions were increased by LPS. Pregabalin improved biochemical, histopathological, and immunohistochemical findings. Conclusion: This study showed that LPS causes pathological findings in the pancreas, but pregabalin has ameliorative effects in aged rats with sepsis. Cas-3, IL-6, iNOS, G-CSF, and SAA all play pivotal roles in the pathogenesis of LPS-induced pancreatic damage.
- Subjects :
- Lipopolysaccharides
0301 basic medicine
Lipopolysaccharide
cell infiltration
Endocrinology, Diabetes and Metabolism
pancreatitis
Anti-Inflammatory Agents
Pregabalin
Nitric Oxide Synthase Type II
Wistar rat
immune response
granulocyte colony stimulating factor
sepsis
Pathogenesis
chemistry.chemical_compound
0302 clinical medicine
biochemical analysis
caspase 3
Nos2 protein, rat
Granulocyte Colony-Stimulating Factor
Pathology
Immunology and Allergy
rat
animal
pancreas
glucose
biology
Caspase 3
lipopolysaccharide
drug effect
single drug dose
Immunohistochemistry
Nitric oxide synthase
female
medicine.anatomical_structure
030220 oncology & carcinogenesis
histopathology
Female
lipids (amino acids, peptides, and proteins)
Inflammation Mediators
Pancreas
antiinflammatory agent
signal transduction
Signal Transduction
medicine.drug
amylase
medicine.medical_specialty
autacoid
cell protection
Casp3 protein, rat
animal experiment
interleukin 6
Granulocyte
Il6 protein, rat
Article
leucocyte infiltration
animal tissue
Sepsis
03 medical and health sciences
Animals
Anti-Inflammatory Agents/*pharmacology
Caspase 3/metabolism
Cytoprotection
Disease Models, Animal
Granulocyte Colony-Stimulating Factor/metabolism
Inflammation Mediators/metabolism
Interleukin-6/metabolism
Nitric Oxide Synthase Type II/metabolism
Pancreas/*drug effects/metabolism/pathology
Pancreatitis/blood/chemically induced/pathology/*prevention & control
Pregabalin/*pharmacology
Rats, Wistar
Serum Amyloid A Protein/metabolism
Signal Transdu
blood
Internal medicine
medicine
Endocrine system
controlled study
neutrophil chemotaxis
protein expression
Serum Amyloid A Protein
nonhuman
Interleukin-6
business.industry
animal model
disease model
aging
inducible nitric oxide synthase
serum amyloid A
triacylglycerol lipase
medicine.disease
030104 developmental biology
Endocrinology
Pancreatitis
chemistry
biology.protein
business
metabolism
Subjects
Details
- ISSN :
- 18715303
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Endocrine, Metabolic & Immune Disorders - Drug Targets
- Accession number :
- edsair.doi.dedup.....23f9114be52657faf470b2e0452280f8