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Molecular epidemiology and characteristic of virulence gene of community-acquired and hospital-acquired methicillin-resistant Staphylococcus aureus isolates in Sun Yat-sen Memorial hospital, Guangzhou, Southern China
- Source :
- BMC Infectious Diseases
- Publisher :
- Springer Nature
-
Abstract
- Background Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of both hospital and community infections globally. It’s important to illuminate the differences between community-acquired MRSA (CA-MRSA) and hospital-acquired MRSA (HA-MRSA), but there have been confusions on the definition, especially for the MRSA isolates identified within 48 h of admission. This study aimed to determine the molecular characteristics and virulence genes profile of CA and HA-MRSA isolates identified less than 48 h after hospital admission in our region. Methods A total 62 MRSA isolates identified within 48 h after admission and the clinical data were collected. Antimicrobial susceptibility test (AST) of collected isolates were performed according to the guidelines of Clinical and Laboratory Standards Institute (CLSI) 2015, and staphylococcal cassette chromosome mec (SCCmec) typing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) and virulence gene profiling were performed to explore the molecular diversity. Results SCCmec III and sequence type (ST) 239 were the most prevalent SCCmec type and ST in both CA and HA-MRSA groups. HA-MRSA group had higher prevalence of SCCmec III (87.2 %) and ST239 (79.5 %) compared with CA-MRSA (60.9 and 43.4 %, both P
- Subjects :
- Adult
Male
Methicillin-Resistant Staphylococcus aureus
0301 basic medicine
China
Adolescent
Virulence Factors
030106 microbiology
Virulence
Microbial Sensitivity Tests
Biology
medicine.disease_cause
Staphylococcal infections
Antimicrobial susceptibility
Microbiology
Young Adult
03 medical and health sciences
0302 clinical medicine
medicine
Pulsed-field gel electrophoresis
Humans
Molecular characteristics
030212 general & internal medicine
Aged
Aged, 80 and over
Molecular Epidemiology
Molecular epidemiology
SCCmec
Middle Aged
Staphylococcal Infections
biochemical phenomena, metabolism, and nutrition
medicine.disease
bacterial infections and mycoses
Methicillin-resistant Staphylococcus aureus
Hospitals
Electrophoresis, Gel, Pulsed-Field
Community-Acquired Infections
Infectious Diseases
Staphylococcus aureus
Virulence gene
Multilocus sequence typing
Female
Research Article
Multilocus Sequence Typing
Subjects
Details
- Language :
- English
- ISSN :
- 14712334
- Volume :
- 16
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Infectious Diseases
- Accession number :
- edsair.doi.dedup.....23dfb9ccc4a79fff77151348eec88265
- Full Text :
- https://doi.org/10.1186/s12879-016-1684-y