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Effects of Cellular Cardiomyoplasty on Ventricular Remodeling Assessed by Doppler Echocardiography and Topographic Immunohistochemistry
- Source :
- Circulation Journal. 68:580-586
- Publication Year :
- 2004
- Publisher :
- Japanese Circulation Society, 2004.
-
Abstract
- Background Myocardial infarction (MI) promotes deleterious remodeling of the myocardium, resulting in ventricular dilation and pump dysfunction. Supplementing infarcted myocardium with neonatal myocyte would attenuate deleterious remodeling and so the present study used Doppler echocardiography and histology to analyze the cardiac function and histological regeneration of the damaged myocardium after cellular cardiomyoplasty. Methods and Results Experimental MI was induced by 24-h coronary ligation followed by reperfusion in adult male Lewis rats and neonatal myocytes were injected directly into the infarct and peri-infarct regions. Three groups of animals were studied at 4 weeks after cellular cardiomyoplasty: noninfarcted control (control), MI plus sham injection (MI), and MI plus cell injection (MI + cell). Ventricular remodeling and cardiac performance were assessed by Doppler echocardiography or contrast echocardiography. At 4 weeks after cellular cardiomyoplasty, MI + cell hearts exhibited attenuation of global ventricular dilation and cardiac function compared with MI hearts not receiving cellular cardiomyoplasty. Immunohistochemically, connexin-43-positive small cells were observed in the vicinity of the infarction in MI + cell heart. By electron microscopy, these cells contained myofilaments with Z-bands and poorly developed intercalated disks, suggesting neonatal myocardial cells. Furthermore, the myocardial cells were often making close contact with interstitial cells. Conclusions Implanted neonatal myocytes form viable grafts after MI, resulting in attenuated ventricular dilation and enhanced contractile function. Echocardiography, electron microscopy, and immunohistochemistry are useful methods for assessing the functional and histological regeneration of the damaged myocardium. (Circ J 2004; 68: 580 - 586)
- Subjects :
- Cardiac function curve
medicine.medical_specialty
Cell Transplantation
Myocardial Infarction
Infarction
Doppler echocardiography
Cell therapy
Internal medicine
Cellular cardiomyoplasty
medicine
Animals
Regeneration
Myocyte
cardiovascular diseases
Myocardial infarction
Cardiomyoplasty
Ventricular remodeling
Muscle Cells
Ventricular Remodeling
medicine.diagnostic_test
business.industry
General Medicine
medicine.disease
Immunohistochemistry
Echocardiography, Doppler
Rats
Actin Cytoskeleton
Disease Models, Animal
Microscopy, Electron
Rats, Inbred Lew
Connexin 43
Heart Function Tests
cardiovascular system
Cardiology
Cardiology and Cardiovascular Medicine
business
Subjects
Details
- ISSN :
- 13474820 and 13469843
- Volume :
- 68
- Database :
- OpenAIRE
- Journal :
- Circulation Journal
- Accession number :
- edsair.doi.dedup.....237d05fa74126dfa9bff688a53b62350