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Oleocanthal Inhibits Catabolic and Inflammatory Mediators in LPS-Activated Human Primary Osteoarthritis (OA) Chondrocytes Through MAPKs/NF-κB Pathways
- Source :
- Cellular Physiology and Biochemistry, Vol 49, Iss 6, Pp 2414-2426 (2018), Cellular Physiology and Biochemistry
- Publication Year :
- 2018
-
Abstract
- Background/Aims: Oleocanthal (OC), a phenolic compound present in extra virgin olive oil (EVOO), has attracted attention since its discovery for its relevant pharmacological properties in different pathogenic processes, including inflammation. Here, we investigated the involvement of OC in LPS-activated osteoarthritis (OA) human primary chondrocytes. Methods: Human primary chondrocytes were harvested from articular cartilage samples obtained from OA patients. The effects of OC on the viability of chondrocytes were tested by MTT assay. Protein and mRNA expression of several catabolic and pro-inflammatory factors after OC treatment were measured by RT-qPCR and western blot respectively. Moreover, we analysed the NO production by Griess reaction. Finally, several pathways mediators were analysed by western blot. Results: We demonstrated that OC did not have any cytotoxic effect. Oleocanthal inhibited NO production and strongly decreased NOS2 and COX-2 protein and mRNA expression in LPS-activated human primary OA chondrocytes. Interestingly, OC also inhibits MMP-13 and ADAMTS-5. In addition, OC downregulates several pro-inflammatory factors, such as IL-6, IL-8, CCL3, LCN2 and TNF-α induced by LPS in human primary OA chondrocytes. Finally, we demonstrated that OC exerts its effects through the MAPK/P38/NF-kB pathways. Conclusion: These data show that OC is able to block LPS-mediated inflammatory response and MMP-13 and ADAMTS-5 induction in human primary OA chondrocytes via MAPKs/NF-kB pathways, suggesting that OC may be a promising agent for the treatment of inflammation in cartilage and a potential molecule to prevent disease progression by inhibiting metalloproteases and aggrecanases.
- Subjects :
- 0301 basic medicine
MAPK/ERK pathway
Lipopolysaccharides
Physiology
p38 mitogen-activated protein kinases
Oleocanthal
CCL3
Nitric Oxide Synthase Type II
Inflammation
Pharmacology
Nitric Oxide
p38 Mitogen-Activated Protein Kinases
lcsh:Physiology
Cyclopentane Monoterpenes
lcsh:Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Chondrocytes
Western blot
Phenols
Matrix Metalloproteinase 13
Osteoarthritis
medicine
Humans
MTT assay
lcsh:QD415-436
Cells, Cultured
Mitogen-Activated Protein Kinase 1
Aldehydes
Mitogen-Activated Protein Kinase 3
medicine.diagnostic_test
lcsh:QP1-981
Chemistry
NF-kappa B
NF-κB
Oleocanthal, Chondrocytes, Phenolic component, Inflammation, Osteoarthritis
030104 developmental biology
Cartilage
Cyclooxygenase 2
Phenolic component
ADAMTS5 Protein
medicine.symptom
Inflammation Mediators
Signal Transduction
Subjects
Details
- ISSN :
- 14219778
- Volume :
- 49
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
- Accession number :
- edsair.doi.dedup.....23595f8aa50c38c907049dc3c81cf246