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Enhanced Bone Augmentation by Controlled Release of Recombinant Human Bone Morphogenetic Protein-2 from Bioabsorbable Membranes
- Source :
- Journal of Periodontology. 74:865-872
- Publication Year :
- 2003
- Publisher :
- Wiley, 2003.
-
Abstract
- 865 Background: The present study was undertaken to determine the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2)-loaded biodegradable membranes on bone augmentation in a rabbit calvarial model. Methods: Five µg of rhBMP-2 was loaded into a stiff hemispherical dome membrane made of poly(L-lactide) and tricalcium phosphate (PLLA/TCP). The release kinetics of rhBMP-2 from the membrane were determined in vitro using a human BMP-2 immunoassay. Twelve rhBMP-2–loaded dome membranes (test group) and 12 control dome membranes (control group) were placed on the partial-thickness calvarial defects of 24 rabbits. The animals were sacrificed at 4 and 8 weeks, and undecalcified ground sections were prepared. Newly formed bone area and height were measured histomorphometrically and calculated by percentage ratio to the total submembranous space area and height below the dome. Results: In vitro release results demonstrated that rhBMP-2 was released consistently over a 4-week period following a high initial burst release on the first day. At both 4 and 8 weeks, histomorphometric analysis revealed that the test group showed significantly higher newly formed bone heights and areas than the control group (P
- Subjects :
- Calcium Phosphates
Polyesters
Bone Morphogenetic Protein 2
chemistry.chemical_element
Biocompatible Materials
Calcium
Bone morphogenetic protein
Bone morphogenetic protein 2
law.invention
Bone augmentation
Random Allocation
Dome (geology)
Drug Delivery Systems
Osteogenesis
Transforming Growth Factor beta
law
Absorbable Implants
Animals
Humans
Wound Healing
Skull
Membranes, Artificial
Anatomy
Controlled release
Recombinant Proteins
Disease Models, Animal
Membrane
chemistry
Delayed-Action Preparations
Bone Morphogenetic Proteins
Recombinant DNA
Periodontics
Rabbits
Bone Diseases
Biomedical engineering
Subjects
Details
- ISSN :
- 19433670 and 00223492
- Volume :
- 74
- Database :
- OpenAIRE
- Journal :
- Journal of Periodontology
- Accession number :
- edsair.doi.dedup.....234f5694cdf285871722690594a06337