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In vivo imaging of in situ motility of fresh and liquid stored ram spermatozoa in the ewe genital tract
- Source :
- Reproduction, Reproduction, 2009, 138 (1), pp.45-53. ⟨10.1530/REP-09-0108⟩, Reproduction, BioScientifica, 2009, 138 (1), pp.45-53. ⟨10.1530/REP-09-0108⟩
- Publication Year :
- 2009
- Publisher :
- Bioscientifica, 2009.
-
Abstract
- The fertility of ram semen after cervical insemination is substantially reduced by 24 h of storage in liquid form. The effects of liquid storage on the transit of ram spermatozoa in the ewe genital tract was investigated using a new procedure allowing direct observation of the spermatozoa in the genital tract. Ejaculated ram spermatozoa were double labeled with R18 and MitoTracker Green FM, and used to inseminate ewes in estrus either cervically through the vagina or laparoscopically into the base of the uterine horns. Four hours after insemination, the spermatozoa were directly observedin situusing fibered confocal fluorescence microscopy in the base, middle and tip of the uterine horns, the utero-tubal junction (UTJ) and the oviduct. The high resolution video images obtained with this technique allowed determination of the distribution of spermatozoa and individual motility in the lumen of the ewe's genital tract. The results showed a gradient of increasing concentration of spermatozoa from the base of the uterus to the UTJ 4 h after intra-uterine insemination into the base of the horns. The UTJ was shown to be a storage region for spermatozoa before their transfer to the oviduct. Thein vitrostorage of spermatozoa in liquid form decreased their migration through the cervix and reduced the proportion of motile spermatozoa and their straight line velocity at the UTJ and their transit into the oviduct.
- Subjects :
- Male
endocrine system
Embryology
Time Factors
[SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging
medicine.medical_treatment
Video Recording
Uterus
Motility
Semen
Biology
Insemination
Andrology
03 medical and health sciences
0302 clinical medicine
Endocrinology
medicine
Animals
Insemination, Artificial
reproductive and urinary physiology
Fluorescent Dyes
030304 developmental biology
Aldehydes
0303 health sciences
Microscopy, Confocal
Sheep
030219 obstetrics & reproductive medicine
urogenital system
Artificial insemination
Reproducibility of Results
Obstetrics and Gynecology
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
Uterine horns
Cell Biology
Spermatozoa
medicine.anatomical_structure
Reproductive Medicine
Sperm Motility
Vagina
Oviduct
Female
Semen Preservation
Subjects
Details
- ISSN :
- 17417899 and 14701626
- Volume :
- 138
- Database :
- OpenAIRE
- Journal :
- REPRODUCTION
- Accession number :
- edsair.doi.dedup.....234e9f0e7c8b524f5c17bddbd85bad4f