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Multi-photon microscopy in cardiovascular research
- Source :
- Methods, 130, 79-89. Elsevier Science
- Publication Year :
- 2017
-
Abstract
- Multiphoton laser scanning microscopy has proven profound value for ex vivo 3D histology and in vivo imaging of motionless tissue. The development of triggering systems and fast imaging methods, combined with advanced preparation procedures solved the challenging task of intravital imaging of the fast pulsating heart and major arteries in animals and further increased the popularity of intravital multiphoton imaging in cardiovascular research.This review article will highlight the potential of multiphoton microscopy for the visualization and characterization of dynamical and structural processes involved in cardiac and vascular diseases, both in an ex vivo and an intravital animal setting. Examples will be given how multiphoton microscopy can be applied to imaging of atherosclerotic plaque development and progression at subcellular level as well as to intravital imaging of inflammatory processes in the heart. In addition to highlighting the potential of multiphoton microscopy in preclinical cardiovascular research, we will discuss how this tool and its applications may be clinically translated to support disease diagnosis and therapy in patients. (C) 2017 Elsevier Inc. All rights reserved.
- Subjects :
- 0301 basic medicine
Biomedical Research
OPTICAL COHERENCE TOMOGRAPHY
ENDOTHELIAL GLYCOCALYX
Cardiovascular research
Nanotechnology
General Biochemistry, Genetics and Molecular Biology
Optical imaging
03 medical and health sciences
Two-photon excitation microscopy
Optical coherence tomography
In vivo
AMERICAN-HEART-ASSOCIATION
2-PHOTON MICROSCOPY
Intravital imaging
Animals
Humans
Medicine
Molecular Biology
IN-VIVO
LASER-SCANNING MICROSCOPY
medicine.diagnostic_test
business.industry
STED microscopy
FLUORESCENCE MICROSCOPY
Intravital Imaging
CONTRAST AGENT
JUNCTIONAL ADHESION MOLECULE
HUMAN CORONARY ATHEROSCLEROSIS
Microscopy, Fluorescence, Multiphoton
030104 developmental biology
Cardiovascular imaging
Cardiovascular Diseases
Non-linear microscopy
business
Preclinical imaging
Ex vivo
Carotid artery
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 10462023
- Database :
- OpenAIRE
- Journal :
- Methods, 130, 79-89. Elsevier Science
- Accession number :
- edsair.doi.dedup.....2349c4f7fe91c06d789fa716032e23ea