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A 60-kDa Plasmodium falciparum protein at the moving junction formed between merozoite and erythrocyte during invasion
- Source :
- Molecular and Biochemical Parasitology. 36:177-185
- Publication Year :
- 1989
- Publisher :
- Elsevier BV, 1989.
-
Abstract
- Invasion of erythrocytes by malaria merozoites requires the formation of a junction of attachment between erythrocyte and merozoite membranes. The attachment junction initially forms at the apical region of the merozoite. It then moves around to the posterior of the merozoite as invasion proceeds. A monoclonal antibody against a 60-kDa merozoite protein (termed MCP-1 for merozoite capping protein 1) of Plasmodium falciparum reacts in an immunofluorescence pattern resembling the moving junction. By two-color immunofluorescence, MCP-1 was located at the attachment site formed between the merozoite apical region and erythrocyte. During invasion, MCP-1 separated and migrated around merozoites at the orifice of the parasitophorous vacuole. In newly-invaded erythrocytes, MCP-1 persisted at the pole of the young parasite nearest the erythrocyte membrane, suggesting its anterior-to-posterior movement. MCP-1 exhibited no variability in molecular mass among the FCR-3, Camp and 7G8 strains of P. falciparum, and the epitope was invariant in the P. falciparum strains studied. We conclude that MCP-1 may participate in merozoite invasion of erythrocytes by facilitating attachment or movement of the junction along the parasite cytoskeletal network.
- Subjects :
- Erythrocytes
biology
medicine.diagnostic_test
Immunoblotting
Plasmodium falciparum
Antibodies, Monoclonal
Fluorescent Antibody Technique
Antigens, Protozoan
biology.organism_classification
Immunofluorescence
Molecular biology
Epitope
Mice
Red blood cell
medicine.anatomical_structure
Cell–cell interaction
Plasmodium knowlesi
medicine
Animals
Protozoa
Parasitology
Cytoskeleton
Molecular Biology
Subjects
Details
- ISSN :
- 01666851
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Molecular and Biochemical Parasitology
- Accession number :
- edsair.doi.dedup.....23419867ccbe57a2f7c22d689c269d58