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The coxsackievirus-adenovirus receptor protein as a cell adhesion molecule in the developing mouse brain
- Source :
- Molecular Brain Research. 77:19-28
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- In an attempt to elucidate the molecular mechanisms underlying neuro-network formation in the developing brain, we analyzed 130 proteolytic cleavage peptides of membrane proteins purified from newborn mouse brains. We describe here the characterization of a membrane protein with an apparent molecular mass of 46 kDa, a member of the immunoglobulin superfamily of which the cDNA sequence was recently reported, encoding the mouse homologue of the human coxsackievirus and adenovirus receptor (mCAR). Western and Northern blot analyses demonstrated the abundant expression of mCAR in the mouse brain, the highest level being observed in the newborn mouse brain, and its expression was detected in embryos as early as at 10. 5 days post-coitus (dpc), but decreased rapidly after birth. On in situ hybridization, mCAR mRNA expression was observed throughout the newborn mouse brain. In primary neurons from the hippocampi of mouse embryos the expression of mCAR was observed throughout the cells including those in growth cones on immunohistochemistry. In order to determine whether or not mCAR is involved in cell adhesion, aggregation assays were carried out. C6 cells transfected with mCAR cDNA aggregated homophilically, which was inhibited by specific antibodies against the extracellular domain of mCAR. In addition to its action as a virus receptor, mCAR may function naturally as an adhesion molecule involved in neuro-network formation in the developing nervous system.
- Subjects :
- Coxsackie and Adenovirus Receptor-Like Membrane Protein
Virus genetics
Transcription, Genetic
Molecular Sequence Data
In situ hybridization
Biology
Transfection
medicine.disease_cause
Hippocampus
Embryonic and Fetal Development
Mice
Cellular and Molecular Neuroscience
Cell Adhesion
medicine
Animals
Humans
Amino Acid Sequence
RNA, Messenger
Northern blot
Cell adhesion
Molecular Biology
Cells, Cultured
Neurons
Cell adhesion molecule
Virus receptor
Cell Membrane
Brain
Gene Expression Regulation, Developmental
Embryo, Mammalian
Molecular biology
Peptide Fragments
Adenoviridae
Animals, Newborn
Organ Specificity
Astrocytes
Receptors, Virus
Immunoglobulin superfamily
Cell Adhesion Molecules
Subjects
Details
- ISSN :
- 0169328X
- Volume :
- 77
- Database :
- OpenAIRE
- Journal :
- Molecular Brain Research
- Accession number :
- edsair.doi.dedup.....233c14f58132581f1b1ddd5480a41838
- Full Text :
- https://doi.org/10.1016/s0169-328x(00)00036-x