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Evaluation of Bifidobacteria and Lactobacillus Probiotics as Alternative Therapy for Salmonella typhimurium Infection in Broiler Chickens

Authors :
Walid Elmonir
Eldsokey Nassef
Mustafa Shukry
Foad Farrag
Tohru Suzuki
Amin Tahoun
May Bin-Jumah
Hanem El-Sharkawy
Mousa O. Germoush
Amira Mohamed Rizk
Ayman M. Mahmoud
Source :
Animals, Volume 10, Issue 6, Animals, Vol 10, Iss 1023, p 1023 (2020), Animals : an Open Access Journal from MDPI
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Chicken Salmonella enterica serovars are enteric bacteria associated with massive public health risks and economic losses. There is a widespread antimicrobial resistance among S. enterica serotypes, and innovative solutions to antibiotic resistance are needed. We aimed to use probiotics to reduce antibiotic resistance and identify the major probiotic players that modify the early interactions between S. enterica and host cells. One-day-old cobb broiler chicks were challenged with S. typhimurium after oral inoculation with different probiotic strains for 3 days. The adherence of different probiotic strains to Caco-2 intestinal epithelial cells was studied in vitro. Lactobacillus (Lacticaseibacillus) casei ATTC334 and Bifidobacterium breve JCM1192 strains attached to Caco-2 cells stronger than B. infantis BL2416. L. casei ATTC334 and B. breve JCM1192 reduced S. typhimurium recovery from the cecal tonsils by competitive exclusion mechanism. Although B. infantis BL2416 bound poorly to Caco-2 epithelial cells, it reduced S. typhimurium recovery and increased IFN-&gamma<br />and TNF-&alpha<br />production. L. casei ATTC334, B. breve JCM1192 and B. infantis BL2416 improved body weight gain and the food conversion rate in S. typhimurium-infected broilers. B. longum Ncc2785 neither attached to epithelial cells nor induced IFN-&gamma<br />release and consequently did not prevent S. typhimurium colonization in broiler chickens. In conclusion, probiotics prevented the intestinal colonization of S. typhimurium in infected chickens by competitive exclusion or cytokine production mechanisms.

Details

ISSN :
20762615
Volume :
10
Database :
OpenAIRE
Journal :
Animals
Accession number :
edsair.doi.dedup.....232d400bf8e2499a1fee38c1ff53a6f7
Full Text :
https://doi.org/10.3390/ani10061023