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Epstein-Barr virus and human papillomavirus serum antibodies define the viral status of nasopharyngeal carcinoma in a low endemic country
- Source :
- Simon, J, Schroeder, L, Ingarfield, K, Diehl, S, Werner, J, Brenner, N, Liu, Z, Pawlita, M, Pring, M, Butt, J, Ness, A & Waterboer, T 2020, ' Epstein-Barr virus and human papillomavirus serum antibodies define the viral status of nasopharyngeal carcinoma in a low endemic country ', International Journal of Cancer, pp. 1-11 . https://doi.org/10.1002/ijc.33006
- Publication Year :
- 2020
-
Abstract
- Epstein-Barr virus (EBV) causes nasopharyngeal carcinoma (NPC) in endemic regions, where almost every tumor is EBV-positive. In Western populations, NPC is rare, and human papillomavirus infection (HPV) has been suggested as another viral cause. We validated multiplex serology with molecular tumor markers, to define EBV-positive, HPV-positive, and EBV-/HPV-negative NPCs in the United Kingdom, and analyzed survival differences between those groups. Sera from NPC cases (N = 98) and age- and sex-matched controls (N = 142) from the Head and Neck 5000 clinical cohort study were analyzed. IgA and IgG serum antibodies against 13 EBV antigens were measured and compared with EBER in situ hybridization (EBER-ISH) data of 41 NPC tumors (29 EBER-ISH positive, 12 negative). IgG antibodies to EBV LF2 correctly diagnosed EBV-positive NPCs in 28 of 29 cases, while all EBER-ISH negative NPCs were seronegative to LF2 IgG (specificity = 100%, sensitivity = 97%). HPV early antigen serology was compared to HPV molecular markers (p16 expression, HPV DNA and RNA) available for 41 NPCs (13 positive, 28 negative). Serology matched molecular HPV markers in all but one case (specificity = 100%, sensitivity = 92%). EBV and HPV infections were mutually exclusive. Overall, 67% of the analyzed NPCs were defined as EBV-positive, 18% as HPV-positive and 14% as EBV/HPV-negative. There was no statistical evidence of a difference in survival between the three groups. These data provide evidence that both, EBV-positive and HPV-positive NPCs are present in a low incidence country, and that EBV and HPV serum antibodies correlate with the viral status of the tumor.
- Subjects :
- Adult
Male
Cancer Research
Epstein-Barr Virus Infections
Herpesvirus 4, Human
In situ hybridization
medicine.disease_cause
Antibodies, Viral
Virus
Serology
03 medical and health sciences
0302 clinical medicine
Antigen
hemic and lymphatic diseases
medicine
otorhinolaryngologic diseases
Humans
Epstein-Barr virus
human papillomavirus
Papillomaviridae
Aged
Nasopharyngeal Carcinoma
biology
business.industry
Incidence (epidemiology)
nasopharyngeal carcinoma
Papillomavirus Infections
virus diseases
Nasopharyngeal Neoplasms
Middle Aged
medicine.disease
Epstein–Barr virus
Virology
Immunoglobulin A
stomatognathic diseases
Oncology
Nasopharyngeal carcinoma
multiplex serology
030220 oncology & carcinogenesis
Case-Control Studies
Immunoglobulin G
biology.protein
Female
Antibody
business
Subjects
Details
- Language :
- English
- ISSN :
- 00207136
- Database :
- OpenAIRE
- Journal :
- Simon, J, Schroeder, L, Ingarfield, K, Diehl, S, Werner, J, Brenner, N, Liu, Z, Pawlita, M, Pring, M, Butt, J, Ness, A & Waterboer, T 2020, ' Epstein-Barr virus and human papillomavirus serum antibodies define the viral status of nasopharyngeal carcinoma in a low endemic country ', International Journal of Cancer, pp. 1-11 . https://doi.org/10.1002/ijc.33006
- Accession number :
- edsair.doi.dedup.....232b54fc3ded71bd4f335fa4eb7fb8d5