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Variable carbon catabolism among Salmonella enterica serovar Typhi isolates
- Source :
- PLoS ONE, Vol 7, Iss 5, p e36201 (2012), PLoS ONE
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- Background Salmonella enterica serovar Typhi (S. Typhi) is strictly a human intracellular pathogen. It causes acute systemic (typhoid fever) and chronic infections that result in long-term asymptomatic human carriage. S. Typhi displays diverse disease manifestations in human infection and exhibits high clonality. The principal factors underlying the unique lifestyle of S. Typhi in its human host during acute and chronic infections remain largely unknown and are therefore the main objective of this study. Methodology/Principal Findings To obtain insight into the intracellular lifestyle of S. Typhi, a high-throughput phenotypic microarray was employed to characterise the catabolic capacity of 190 carbon sources in S. Typhi strains. The success of this study lies in the carefully selected library of S. Typhi strains, including strains from two geographically distinct areas oftyphoid endemicity, an asymptomatic human carrier, clinical stools and blood samples and sewage-contaminated rivers. An extremely low carbon catabolic capacity (27% of 190 carbon substrates) was observed among the strains. The carbon catabolic profiles appeared to suggest that S. Typhi strains survived well on carbon subtrates that are found abundantly in the human body but not in others. The strains could not utilise plant-associated carbon substrates. In addition, α-glycerolphosphate, glycerol, L-serine, pyruvate and lactate served as better carbon sources to monosaccharides in the S. Typhi strains tested. Conclusion The carbon catabolic profiles suggest that S. Typhi could survive and persist well in the nutrient depleted metabolic niches in the human host but not in the environment outside of the host. These findings serve as caveats for future studies to understand how carbon catabolism relates to the pathogenesis and transmission of this pathogen.
- Subjects :
- Bacterial Diseases
Glycerol
Applied Microbiology
Carboxylic Acids
lcsh:Medicine
Salmonella typhi
Biochemistry
Pathogenesis
Salmonella
Microbial Physiology
Pyruvic Acid
Serine
Cluster Analysis
lcsh:Science
Pathogen
Multidisciplinary
Transmission (medicine)
Microbial Growth and Development
Nucleosides
Bacterial Pathogens
Infectious Diseases
Phenotype
Medical Microbiology
Glycerophosphates
Metabolome
Carbohydrate Metabolism
Medicine
Research Article
Biology
Microbiology
complex mixtures
Typhoid fever
Typhus
medicine
Lactic Acid
Microbial Pathogens
Microbial Metabolism
Catabolism
Host (biology)
lcsh:R
medicine.disease
Virology
Carbon
Metabolism
lcsh:Q
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....2317d140a3e2a95b3484c4d248bafd91