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Benzo[a]pyrene up-regulates cyclooxygenase-2 gene expression in oral epithelial cells
- Source :
- Carcinogenesis. 18:795-799
- Publication Year :
- 1997
- Publisher :
- Oxford University Press (OUP), 1997.
-
Abstract
- Cyclooxygenase may be important in the pathogenesis of smoking-related cancer because it activates carcinogens and catalyzes prostaglandin biosynthesis. We determined the effects of benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon in tobacco smoke, on cyclooxygenase-2 (Cox-2) mRNA, protein and synthesis of prostaglandin E2 (PGE2) in normal and transformed oral epithelial cells. Treatment with B[a]P caused a dose-dependent increase in production of PGE2, with a maximal increase of approximately 100%. Enhanced synthesis of PGE2 was associated with increased amounts of Cox-2 protein. B[a]P also caused a two-fold increase in Cox-2 mRNA in both normal and transformed cells. Transient transfections with a Cox-2 promoter construct showed that B[a]P-mediated induction of Cox-2 mRNA reflected increased transcription. Levels of Cox-1 were unaffected by B[a]P. B[e]P did not affect the synthesis of PGE2 or amounts of Cox-2. These data are important because B[a]P-mediated induction of Cox-2 may predispose to carcinogenesis by enhancing the production of mutagens and the synthesis of prostaglandins.
- Subjects :
- Cancer Research
medicine.disease_cause
Dinoprostone
Gene Expression Regulation, Enzymologic
chemistry.chemical_compound
Gene expression
Benzo(a)pyrene
medicine
Humans
Prostaglandin E2
Cells, Cultured
Carcinogen
Messenger RNA
biology
Chemistry
Mouth Mucosa
Membrane Proteins
General Medicine
Transfection
Molecular biology
Isoenzymes
Biochemistry
Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
Enzyme Induction
biology.protein
Cyclooxygenase
Carcinogenesis
medicine.drug
Subjects
Details
- ISSN :
- 14602180
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Carcinogenesis
- Accession number :
- edsair.doi.dedup.....23109da83799953761cdf9d9ccb4bdba