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Soft x ray absorption spectroscopy of metalloproteins and high valent metal complexes at room temperature using free electron lasers
- Source :
- Structural Dynamics, Vol 4, Iss 5, Pp 054307-054307-16 (2017), Structural Dynamics, Kubin, M; Kern, J; Gul, S; Kroll, T; Chatterjee, R; Löchel, H; et al.(2017). Soft x-ray absorption spectroscopy of metalloproteins and high-valent metal-complexes at room temperature using free-electron lasers. Structural Dynamics, 4(5). doi: 10.1063/1.4986627. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/2xh3s283, Structural dynamics (Melville, N.Y.), vol 4, iss 5
- Publication Year :
- 2017
-
Abstract
- X-ray absorption spectroscopy at the L-edge of 3d transition metals provides unique information on the local metal charge and spin states by directly probing 3d-derived molecular orbitals through 2p-3d transitions. However, this soft x-ray technique has been rarely used at synchrotron facilities for mechanistic studies of metalloenzymes due to the difficulties of x-ray-induced sample damage and strong background signals from light elements that can dominate the low metal signal. Here, we combine femtosecond soft x-ray pulses from a free-electron laser with a novel x-ray fluorescence-yield spectrometer to overcome these difficulties. We present L-edge absorption spectra of inorganic high-valent Mn complexes (Mn ∼ 6–15 mmol/l) with no visible effects of radiation damage. We also present the first L-edge absorption spectra of the oxygen evolving complex (Mn4CaO5) in Photosystem II (Mn
- Subjects :
- Free electron model
Spin states
Absorption spectroscopy
Analytical chemistry
02 engineering and technology
010402 general chemistry
Experimental Methodologies
Physical Chemistry
01 natural sciences
Metal
ARTICLES
Transition metal
lcsh:QD901-999
Molecular orbital
Spectroscopy
Instrumentation
Fysikalisk kemi
X-ray spectroscopy
Radiation
Chemistry
Institut für Physik und Astronomie
021001 nanoscience & nanotechnology
Condensed Matter Physics
0104 chemical sciences
visual_art
visual_art.visual_art_medium
Condensed Matter::Strongly Correlated Electrons
Inhouse research on structure dynamics and function of matter
lcsh:Crystallography
0210 nano-technology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Structural Dynamics, Vol 4, Iss 5, Pp 054307-054307-16 (2017), Structural Dynamics, Kubin, M; Kern, J; Gul, S; Kroll, T; Chatterjee, R; Löchel, H; et al.(2017). Soft x-ray absorption spectroscopy of metalloproteins and high-valent metal-complexes at room temperature using free-electron lasers. Structural Dynamics, 4(5). doi: 10.1063/1.4986627. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/2xh3s283, Structural dynamics (Melville, N.Y.), vol 4, iss 5
- Accession number :
- edsair.doi.dedup.....22f5b978a0ecc953cce6c36be3b3df25