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Virucidal effect of monogalactosyl diacylglyceride from a green microalga, Coccomyxa sp. KJ, against clinical isolates of SARS‐CoV‐2 as assessed by a plaque assay
- Source :
- Journal of Clinical Laboratory Analysis
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Background Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is the causative agent of coronavirus disease 2019 (COVID‐19) and is capable of human‐to‐human transmission and rapid global spread. Thus, the establishment of high‐quality viral detection and quantification methods, and the development of anti‐SARS‐CoV‐2 agents are critical. Methods Here, we present the rapid detection of infectious SARS‐CoV‐2 particles using a plaque assay with 0.5% agarose‐ME (Medium Electroosmosis) as an overlay medium. Results The plaques were capable of detecting the virus within 36–40 h post‐infection. In addition, we showed that a monogalactosyl diacylglyceride isolated from a microalga (Coccomyxa sp. KJ) could inactivate the clinical isolates of SARS‐CoV‐2 in a time‐ and concentration‐dependent manner. Conclusions These results would allow rapid quantification of the infectious virus titers and help develop more potent virucidal agents against SARS‐CoV‐2.<br />We presented the rapid detection of SARS‐CoV‐2 using a plaque assay within 36–40 h post‐infection. In addition, we showed that a compound from a microalga inactivated the virus.
- Subjects :
- Microbiology (medical)
natural product
Coronavirus disease 2019 (COVID-19)
viruses
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Clinical Biochemistry
virucidal action
Viral Plaque Assay
Biology
Antiviral Agents
Rapid detection
Virus
Glycerides
Microbiology
Chlorophyta
Chlorocebus aethiops
Microalgae
Animals
Humans
Immunology and Allergy
Vero Cells
Research Articles
Infectious virus
Virus quantification
SARS-CoV-2
Biochemistry (medical)
Public Health, Environmental and Occupational Health
COVID-19
Galactose
virus diseases
Hematology
biology.organism_classification
quantification
Medical Laboratory Technology
Titer
detection time
Coccomyxa
infectious virus
Research Article
Subjects
Details
- ISSN :
- 10982825 and 08878013
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Laboratory Analysis
- Accession number :
- edsair.doi.dedup.....22efc47fc2a356d2cb7d057cece572e4