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Presence of peptide synthetase gene transcripts and accumulation of ergopeptines in Claviceps purpurea and Neotyphodium coenophialum
- Source :
- Canadian journal of microbiology. 44(1)
- Publication Year :
- 1998
-
Abstract
- The production of toxic ergopeptine alkaloids by the fungi Claviceps purpurea and Neotyphodium coenophialum involves the activity of one or more nonribosomal peptide synthetases. Claviceps purpurea and N. coenophialum each have several different peptide synthetase genes, fragments of which have been cloned previously. An additional Claviceps purpurea peptide synthetase gene was cloned by hybridization with one of the N. coenophialum peptide synthetase gene fragments. We detected the presence of mRNA from the peptide synthetase genes in cultures of different ages grown under conditions favorable or unfavorable for ergopeptine production. All four peptide synthetase genes from Claviceps purpurea were transcribed under at least some of the experimental conditions. Transcripts from three of the four genes were detected under conditions consistent with their potential involvement in ergopeptine biosynthesis. All three peptide synthetase genes previously identified in N. coenophialum were transcribed during symbiotic growth of this fungus with tall fescue, as well as in ergopeptine-producing cultures. The data show that all of the peptide synthetase genes are transcribed, that one of the peptide synthetase genes is dissociated from ergopeptine biosynthesis, and, as a result, prioritize the remaining genes for functional analyses by transformation-mediated gene disruption.Key words: Claviceps purpurea, endophyte, ergot, ergotamine, Neotyphodium coenophialum.
- Subjects :
- Immunology
Molecular Sequence Data
Peptide
Poaceae
Applied Microbiology and Biotechnology
Microbiology
Claviceps
chemistry.chemical_compound
Biosynthesis
Nonribosomal peptide
Genetics
Ergotamine
RNA, Messenger
Cloning, Molecular
Peptide Synthases
Symbiosis
Molecular Biology
Gene
chemistry.chemical_classification
Messenger RNA
biology
RNA, Fungal
General Medicine
Neotyphodium
biology.organism_classification
Claviceps purpurea
Enzyme
chemistry
Biochemistry
Hypocreales
Oligopeptides
Subjects
Details
- ISSN :
- 00084166
- Volume :
- 44
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Canadian journal of microbiology
- Accession number :
- edsair.doi.dedup.....22e41cbda65801b56016b67a7ae9272d