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Combined utilization of nutrients and sugar derived from wheat bran for d-Lactate fermentation by Sporolactobacillus inulinus YBS1-5
- Source :
- Bioresource Technology. 229:33-38
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- To decrease d-Lactate production cost, wheat bran, a low-cost waste of milling industry, was selected as the sole feedstock. First, the nutrients were recovered from wheat bran by acid protease hydrolysis. Then, cellulosic hydrolysates were prepared from protease-treated samples after acid pretreatment and enzymatic saccharification. The combined use of nutrients and hydrolysates as nitrogen and carbon sources for fermentation by S. inulinus YB1-5 resulted in d-Lactate levels of 99.5g/L, with an average production efficiency of 1.94g/L/h and a yield of 0.89g/g glucose. Moreover, fed-batch simultaneous saccharification and fermentation process at 40°C, 20% (w/v) solid loading and 20FPU/g solid cellulase concentration was obtained. d-Lactate concentrations, yield, productivity, and optical purity were 87.3g/L, 0.65g/g glucose, 0.81g/L/h and 99.1%, respectively. This study provided a feasible procedure that can help produce cellulosic d-Lactate using agricultural waste without external nutrient supplementation.
- Subjects :
- Dietary Fiber
0106 biological sciences
Time Factors
Environmental Engineering
Carbohydrates
Bioengineering
Cellulase
010501 environmental sciences
Raw material
01 natural sciences
Hydrolysate
Hydrolysis
010608 biotechnology
Lactic Acid
Food science
Sugar
Waste Management and Disposal
0105 earth and related environmental sciences
Bacillales
Bran
biology
Renewable Energy, Sustainability and the Environment
Chemistry
food and beverages
General Medicine
Batch Cell Culture Techniques
Cellulosic ethanol
Fermentation
Costs and Cost Analysis
biology.protein
Carbohydrate Metabolism
Peptide Hydrolases
Subjects
Details
- ISSN :
- 09608524
- Volume :
- 229
- Database :
- OpenAIRE
- Journal :
- Bioresource Technology
- Accession number :
- edsair.doi.dedup.....22dfadea962d0e24d9bcdc3b6db5344f