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Neutralizing antibody response of rabbits and goats to caprine arthritis-encephalitis virus
- Source :
- Infection and Immunity. 38:455-461
- Publication Year :
- 1982
- Publisher :
- American Society for Microbiology, 1982.
-
Abstract
- Rabbits were immunized with purified caprine arthritis-encephalitis virus and examined for neutralizing activity. Analysis of virus-antiserum interaction at 37 degrees C demonstrated little loss of viral infectivity after incubation with heat-inactivated rabbit antiserum for 60 min. However, sensitization of virus (as assessed by the addition of complement) occurred almost immediately and was 95% complete after 10 min. The complement-dependent neutralizing activity was associated with the immunoglobulin G fraction of rabbit antiserum. Addition of goat anti-rabbit immunoglobulin G to the immune rabbit serum-caprine arthritis-encephalitis virus mixture also resulted in neutralization of infectivity when unbound antibody was removed before addition of the anti-immunoglobulin. Serum from most caprine arthritis-encephalitis virus-infected goats contains antibody activity to the core protein p28, as demonstrated by immunodiffusion and enzyme-linked immunosorbent assay. However, attempts to demonstrate neutralizing activity in the serum of goats up to 1.5 years post-inoculation or in serum of hyperimmunized goats were unsuccessful when the sera were examined alone or in combination with complement or rabbit anti-goat immunoglobulin or both.
- Subjects :
- Immunology
Antibodies, Viral
Microbiology
Neutralization
Immunoglobulin G
Virus
Neutralization Tests
Animals
Neutralizing antibody
Caprine arthritis encephalitis virus
Infectivity
biology
Goats
Complement System Proteins
biology.organism_classification
Virology
Antibodies, Anti-Idiotypic
Immunodiffusion
Retroviridae
Infectious Diseases
biology.protein
Immunization
Parasitology
Rabbits
Antibody
Retroviridae Infections
Research Article
Subjects
Details
- ISSN :
- 10985522 and 00199567
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....22dbe41fa7851e6397adf4f881047425