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Variations of DNA methylation in Eucalyptus urophylla×Eucalyptus grandis shoot tips and apical meristems of different physiological ages
- Source :
- Physiologia Plantarum, Physiologia Plantarum, Wiley, 2011, 143 (2), pp.178-187. ⟨10.1111/j.1399-3054.2011.01491.x⟩
- Publication Year :
- 2011
- Publisher :
- HAL CCSD, 2011.
-
Abstract
- International audience; Global DNA methylation was assessed by high-performance liquid chromatography (HPLC) for the first time in Eucalyptus urophylla×Eucalyptus grandis shoot tips comparing three outdoor and one in vitro sources of related genotypes differing in their physiological age. The DNA methylation levels found were consistent with those reported for other Angiosperms using the same HPLC technology. Notwithstanding noticeable time-related fluctuations within each source of plant material, methylation rate was overall higher for the mature clone (13.7%) than for the rejuvenated line of the same clone (12.6%) and for the juvenile offspring seedlings (11.8%). The in vitro microshoots of the mature clone were less methylated (11.3%) than the other outdoor origins, but the difference with the juvenile seedlings was not significant. Immunofluorescence investigations on shoot apices established that the mature source could be distinguished from the rejuvenated and juvenile origins by a higher density of cells with methylated nuclei in leaf primordia. Shoot apical meristems (SAMs) from the mature clone also showed a greater proportion and more methylated cells than SAMs from the rejuvenated and juvenile origins. The nuclei of these latter were characterized by fewer and more dispersed labeled spots than for the mature source. Our findings establish that physiological ageing induced quantitative and qualitative variations of DNA methylation at shoot tip, SAM and even cellular levels. Overall this DNA methylation increased with maturation and conversely decreased with rejuvenation to reach the lower scores and to show the immunolabeling patterns that characterized juvenile material nuclei.
- Subjects :
- 0106 biological sciences
Eucalyptus grandis
Chromatographic (HPLC)
DNA, Plant
Genotype
Physiology
Meristem
Clone (cell biology)
rejuvenation
Fluorescent Antibody Technique
genotypr
Immunofluorescence investigations
F62 - Physiologie végétale - Croissance et développement
Plant Science
Biology
01 natural sciences
F30 - Génétique et amélioration des plantes
03 medical and health sciences
Botany
Genetics
Image Processing, Computer-Assisted
Juvenile
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Primordium
Chromatography, High Pressure Liquid
030304 developmental biology
Cell Nucleus
0303 health sciences
Eucalyptus
DNA methylation
food and beverages
Cell Biology
General Medicine
Methylation
Eucalyptus urophylla
Plant Leaves
SAM
Ageing
Seedlings
Shoot
Shoot apical meristems
juvenile material nuclei
Plant Shoots
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 00319317 and 13993054
- Database :
- OpenAIRE
- Journal :
- Physiologia Plantarum, Physiologia Plantarum, Wiley, 2011, 143 (2), pp.178-187. ⟨10.1111/j.1399-3054.2011.01491.x⟩
- Accession number :
- edsair.doi.dedup.....22cbce261f83250ef4192195465631eb