Additional file 1 of Circular RNA CRIM1 functions as a ceRNA to promote nasopharyngeal carcinoma metastasis and docetaxel chemoresistance through upregulating FOXQ1

Additional file 1: Figure S1. Schematic map of circRNA isoforms arising from CRIM1 genome and relative circCRIM1 expression in NPC cells and tissues. Figure S2. Knockdown and overexpression efficiency of circCRIM1 or miR-422a in NPC cells. Figure S3–4. Multiple sequence alignment of two independent siRNAs targeting circCRIM1 and 15 circRNA isoforms derived from CRIM1 gene in S18 cells. Figure S5. Transwell assays for the rescue effects of circCRIM1 with a mutant backsplice junction of circCRIM1. Figure S6. CircCRIM1 induces EMT in NPC cells. Figure S7. FOXQ1 is a downstream target of miR-422a and circCRIM1 involved in metastasis. Figure S8. FOXQ1 downregulation inhibited NPC cell metastasis in vivo. Figure S9. circCRIM1 downregulation enhances the docetaxel efficacy on NPC cell metastasis in vivo. Figure S10. Patients in the combined intermediate and high risk groups did not benefit from docetaxel-containing induction chemotherapy. Figure S11. Full unedited Western blotting gels for all figures. Table S1: List of 15 circRNA isoforms derived from CRIM1 gene in S18 cells. Table S2. Downregulated miRNAs according to miRNA microarray. Table S3. Putative miRNAs predicted to bind circCRIM1 by miRanda and RNAhybrid algorithms. Table S4. Putative miRNAs predicted to bind circCRIM1 by StarBase algorithms. Table S5. Clinical characteristics of NPC patients according to high and low circCRIM1 expression. Table S6. Univariate and multivariable Cox regression analysis of circCRIM1 expression level and survival in NPC patients. Table S7. Primers and RNA sequences used in this study.