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Expression and function of the angiopoietin receptor Tie-2 in human eosinophils
- Source :
- Journal of Allergy and Clinical Immunology. 114:1077-1084
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- Background Increased vascularity of bronchial mucosa is closely related to the expression of angiogenic factors, which contribute to the pathogenesis of diseases such as asthma bronchiale. Objective Here we examine the effects of the angiogenic growth factors angiopoietin 1 and angiopoietin 2 on eosinophil function in vitro and possible involvement of the angiopoietin receptor Tie-2. Methods Eosinophil migration was studied by micropore filter assays. Signaling mechanisms required for angiopoietin-dependent migration were tested by using signaling enzyme blockers. Tie-2 mRNA and receptor expression on the cell surface of eosinophils was demonstrated in RT-PCR and by fluorescence-activated cell sorting analysis. Results Angiopoietin 1 significantly stimulated eosinophil chemotaxis via activation of phosphodiesterase, phosphatidylinositol 3′-kinase, and tyrosine kinases. The effect on eosinophil migration of angiopoietin 1 was reversed by an antibody against the Tie-2 receptor and by angiopoietin 2. Incubation of eosinophils with angiopoietin 1 abolished the chemotactic effects of vascular endothelial growth factor on human eosinophils via the Tie-2 receptor. Finally, Tie-2 expression by human eosinophils was demonstrated on the transcriptional and protein level. Conclusions Data suggest that angiopoietin 1 stimulates directed migration and possibly inhibits vascular endothelial growth factor–induced eosinophil chemotaxis via its Tie-2 receptor, which is expressed by eosinophils. Thus, angiopoietin 1 may play an important role in the modulation of eosinophilic inflammation.
- Subjects :
- Vascular Endothelial Growth Factor A
medicine.medical_specialty
Receptor expression
Immunology
Angiopoietin-2
Angiopoietin
Phosphatidylinositol 3-Kinases
chemistry.chemical_compound
Eosinophil migration
Cell Movement
1-Methyl-3-isobutylxanthine
Internal medicine
Angiopoietin-1
medicine
Humans
Immunology and Allergy
RNA, Messenger
biology
Chemotaxis
respiratory system
Eosinophil
Receptor, TIE-2
Angiopoietin receptor
Cell biology
Eosinophils
Vascular endothelial growth factor
Endocrinology
medicine.anatomical_structure
chemistry
cardiovascular system
biology.protein
Eosinophil chemotaxis
Tyrosine kinase
Subjects
Details
- ISSN :
- 00916749
- Volume :
- 114
- Database :
- OpenAIRE
- Journal :
- Journal of Allergy and Clinical Immunology
- Accession number :
- edsair.doi.dedup.....22c093e0ba7cbb0c4a4c03b4d946aca9
- Full Text :
- https://doi.org/10.1016/j.jaci.2004.06.045