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Cell Origami: Self-Folding of Three-Dimensional Cell-Laden Microstructures Driven by Cell Traction Force
- Source :
- PLoS ONE, PLoS ONE, Vol 7, Iss 12, p e51085 (2012)
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- This paper describes a method of generating three-dimensional (3D) cell-laden microstructures by applying the principle of origami folding technique and cell traction force (CTF). We harness the CTF as a biological driving force to fold the microstructures. Cells stretch and adhere across multiple microplates. Upon detaching the microplates from a substrate, CTF causes the plates to lift and fold according to a prescribed pattern. This self-folding technique using cells is highly biocompatible and does not involve special material requirements for the microplates and hinges to induce folding. We successfully produced various 3D cell-laden microstructures by just changing the geometry of the patterned 2D plates. We also achieved mass-production of the 3D cell-laden microstructures without causing damage to the cells. We believe that our methods will be useful for biotechnology applications that require analysis of cells in 3D configurations and for self-assembly of cell-based micro-medical devices.
- Subjects :
- Anatomy and Physiology
Cell traction
Materials Science
Biomedical Engineering
Hinge
lcsh:Medicine
Bioengineering
Nanotechnology
Material requirements
Medical Devices
Mice
Engineering
Molecular Cell Biology
Cell Mechanics
Animals
Biomechanics
lcsh:Science
Cell shape
Biology
Musculoskeletal System
Microstructure
Cell Shape
Cytoskeleton
Physics
Multidisciplinary
Tissue Engineering
Mechanical Engineering
Self folding
lcsh:R
Biocompatible material
Cellular Structures
Lift (force)
NIH 3T3 Cells
Microtechnology
lcsh:Q
Research Article
Biotechnology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....22b41ef97af66714cd7cbea433786055