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Detection and Interpretation of Lysergic Acid Diethylamide Results by Immunoassay Screening of Urine in Various Testing Groups
- Source :
- Journal of Analytical Toxicology. 21:181-184
- Publication Year :
- 1997
- Publisher :
- Oxford University Press (OUP), 1997.
-
Abstract
- A total of 2259 urine samples were assayed for lysergic acid diethylamide (LSD) using radioimmunoassay (RIA, Coat-a-Count, Diagnostics Products) and a premarket cloned enzyme donor immunoassay (CEDIA, Boehringer Mannheim). Urine samples were obtained from patients admitted to the emergency room, patients in drug rehabilitation programs, and adults and juveniles in criminal probation programs. An overall incidence of positive results was 0.80% for CEDIA (500-pg/mL cutoff) and 0.89% and 0.18% for RIA at cutoffs of 250 and 500 pg/mL, respectively. Of the CEDIA-positive samples, only 17 and 11% were positive by RIA at 250 and 500 pg/mL, respectively, whereas among RIA-positive samples, only 10% of those > 250 pg/mL and only 25% of those > 500 pg/mL were positive by CEDIA. Moreover, only 2 of 25 of samples positive by one of these screening assays were confirmed by gas chromatography-mass spectrometry (GC-MS). It is likely that discrepancies in results between immunoassays are due to differences in antibody specificities used to detect LSD metabolites. In addition, immunoassays may be more sensitive than GC-MS for detecting LSD use as current confirmation assays are targeted towards detection of the parent drug only. The interpretation of results for LSD analysis must be made with knowledge of the limitations for each assay.
- Subjects :
- Substance-Related Disorders
Health, Toxicology and Mutagenesis
Radioimmunoassay
Urine
Cross Reactions
Toxicology
Gas Chromatography-Mass Spectrometry
Biological fluid
Analytical Chemistry
Immunoenzyme Techniques
Antibody Specificity
Reference Values
Prevalence
medicine
Humans
Environmental Chemistry
Routine analysis
Lysergic acid diethylamide
Chemical Health and Safety
Chromatography
medicine.diagnostic_test
Chemistry
Incidence
Reproducibility of Results
Ergot Derivatives
Lysergic Acid Diethylamide
Immunoassay
Hallucinogens
Substance Abuse Treatment Centers
Emergency Service, Hospital
Quantitative analysis (chemistry)
medicine.drug
Subjects
Details
- ISSN :
- 19452403 and 01464760
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Journal of Analytical Toxicology
- Accession number :
- edsair.doi.dedup.....22abca0ab6c97eb008b75b124db0094e