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Determination of Selected Isoquinoline Alkaloids from Mahonia aquifolia; Meconopsis cambrica; Corydalis lutea; Dicentra spectabilis; Fumaria officinalis; Macleaya cordata Extracts by HPLC-DAD and Comparison of Their Cytotoxic Activity
- Source :
- Toxins, Vol 11, Iss 10, p 575 (2019), Toxins
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Alkaloids have protective functions for plants and can play an important role in living organisms. Alkaloids may have a wide range of pharmacological activities. Many of them have cytotoxic activity. Nowadays, cancer has become a serious public health problem. Searching for effective drugs with anticancer activity is one of the most significant challenges of modern scientific research. The aim of this study was the investigation of cytotoxic activity of extracts obtained from Corydalis lutea root and herb, Dicentra spectabilis root and herb, Fumaria officinalis, Macleaya cordata leaves and herb, Mahonia aquifolia leaves and cortex, Meconopsis cambrica root and herb on FaDu, SCC-25, MCF-7, and MDA-MB-231 cancer cell lines. The cytotoxic activity of these extracts has not been previously tested for these cell lines. The aim was also to quantify selected alkaloids in the investigated extracts by High Performance Liquid Chromatography (HPLC). The analyses of alkaloid content were performed using HPLC in reversed phase (RP) mode using Polar RP column and mobile phase containing acetonitrile, water and ionic liquid (IL). Cytotoxic effect of the tested plant extracts and respective alkaloid standards were examined using human pharyngeal squamous carcinoma cells (FaDu), human tongue squamous carcinoma cells (SCC-25), human breast adenocarcinoma cell line (MCF-7), human triple-negative breast adenocarcinoma cell line (MDA-MB-231). All investigated plant extracts possess cytotoxic activity against tested cancer cell lines: FaDu, SCC-25, MCF-7, and MDA-MB-231. The highest cytotoxic activity against FaDu, SCC-25, and MCF-7 cell lines was estimated for Macleaya cordata leaf extract, while the highest cytotoxic activity against MDA-MB-231 cell line was obtained for Macleaya cordata herb extract. Differences in cytotoxic activity were observed for extracts obtained from various parts of investigated plants. In almost all cases the cytotoxic activity of investigated plant extracts, especially at the highest concentration against tested cell lines was significantly higher than the activity of anticancer drug etoposide. Our investigations exhibit that these plant extracts can be recommended for further in vivo experiments to confirm their anticancer activity.
- Subjects :
- corydalis lutea
Cell Survival
Health, Toxicology and Mutagenesis
Meconopsis cambrica
Mahonia
lcsh:Medicine
Antineoplastic Agents
Toxicology
macleaya cordata
01 natural sciences
Article
Corydalis lutea
Macleaya cordata
Magnoliopsida
03 medical and health sciences
Alkaloids
0302 clinical medicine
Tandem Mass Spectrometry
Cell Line, Tumor
Humans
meconopsis cambrica
Mahonia aquifolia
Medicinal plants
Chromatography, High Pressure Liquid
Cell Proliferation
cytotoxic activity
Fumaria officinalis
biology
Traditional medicine
Plant Extracts
Chemistry
Alkaloid
010401 analytical chemistry
lcsh:R
Corydalis
fumaria officinalis
Isoquinolines
biology.organism_classification
Dicentra spectabilis
0104 chemical sciences
Squamous carcinoma
mahonia aquifolia
030220 oncology & carcinogenesis
dicentra spectabilis
hplc-dad
isoquinoline alkaloids
Subjects
Details
- Language :
- English
- ISSN :
- 20726651
- Volume :
- 11
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Toxins
- Accession number :
- edsair.doi.dedup.....22a0535f120d6d1459c8bd1ff0d85c42