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Resveratrol Arrests the Cell Division Cycle at S/G2 Phase Transition
- Source :
- Biochemical and Biophysical Research Communications. 250:53-58
- Publication Year :
- 1998
- Publisher :
- Elsevier BV, 1998.
-
Abstract
- Resveratrol (3,5,4′-trihydroxystilbene) is a naturally occurring phytoalexin, found in grapes and wine, which has been reported to exert a variety of important pharmacological effects. We have investigated the activity of resveratrol on proliferation and differentiation of the promyelocitic cell line HL-60. A concentration as low as 30 μM causes a complete arrest of proliferation and a rapid induction of differentiation towards a myelo-monocytic phenotype. Analyses by flow cytometry showed the absence of the G2/M peak and the accumulation of cells in G1 and S phases. Moreover, at the concentrations employed, a very low amount of apoptotic cells was evidenced. A detailed biochemical analysis demonstrated that the G1 phase of the cell division cycle engine was completely unmodified by resveratrol addition, thus indicating that the G1 → S transition occurs normally. Conversely, after only 24 h treatment, a significant increase of cyclins A and E could be observed along with the accumulation of cdc2 in the inactive phosphorylated form. These data demonstrate that resveratrol causes a complete and reversible cell cycle arrest at the S phase checkpoint.
- Subjects :
- DNA Replication
G2 Phase
Cell cycle checkpoint
Biophysics
HL-60 Cells
Biology
Resveratrol
Biochemistry
S Phase
Flow cytometry
chemistry.chemical_compound
Stilbenes
medicine
Anticarcinogenic Agents
Humans
Molecular Biology
Cyclin
chemistry.chemical_classification
Cyclin-dependent kinase 1
medicine.diagnostic_test
Phytoalexin
food and beverages
Cell Differentiation
Cell Biology
Cell biology
chemistry
Apoptosis
Cell culture
Cell Division
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 250
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....22998551e222cfa65a63af69558d9cf6