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Selenium-enriched polysaccharides from Pyracantha fortuneana (Se-PFPs) inhibit the growth and invasive potential of ovarian cancer cells through inhibiting β-catenin signaling
- Source :
- Oncotarget
- Publication Year :
- 2015
-
Abstract
- Polysaccharides from medicinal plants exert antitumor activity in many cancers. Our previous study demonstrated that polysaccharides extracted from the selenium-enriched Pyracantha fortuneana (Se-PFPs) showed antiproliferative effect in breast cancer cell line. This study aimed to investigate the antitumor effect of Se-PFPs in ovarian cancer cells in vitro and in vivo. Se-PFPs could decrease cell viability, induce apoptosis, and inhibit migratory and invasive potentials in HEY and SKOV3 cells. These findings are supported by reduced expression of cyclin D1, Bcl-2 and MMP-9, enhanced cleavage of PARP and caspase-3, elevated activity of caspase-3 and caspase-9, and EMT (epithelial to mesenchymal transition) inhibition (elevated expression of E-cadherin and cytokeratin 19, and reduced expression of N-cadherin, vimentin, ZEB1 and ZEB2). Moreover, Se-PFPs inhibited xenografted tumor growth through inhibiting cell proliferation and inducing cell apoptosis. More importantly, Se-PFPs significantly reduced cytoplasmic β-catenin particularly nuclear β-catenin expression but increased β-catenin phosphorylation in a GSK-3β-dependent mechanism. Furthermore, β-catenin knockdown exerted similar effects on cell proliferation and invasion as seen in Se-PFPs-treated cells, while β-catenin overexpression neutralized the inhibitory effects of Se-PFPs on cell proliferation and invasion. Take together,Se-PFPs exert antitumor activity through inhibiting cell proliferation, migration, invasion and EMT, and inducing cell apoptosis. These effects are achieved by the inhibition of β-catenin signaling. Thus Se-PFPs can be used as potential therapeutic agents in the prevention and treatment of ovarian cancer.
- Subjects :
- 0301 basic medicine
Beta-catenin
Epithelial-Mesenchymal Transition
Mice, Nude
Vimentin
Antineoplastic Agents
Apoptosis
03 medical and health sciences
Mice
0302 clinical medicine
Cyclin D1
Cell Movement
Polysaccharides
Cell Line, Tumor
selenium-enriched polysaccharides
medicine
Animals
Humans
Neoplasm Invasiveness
antitumor activity
Viability assay
Epithelial–mesenchymal transition
Selenium Compounds
beta Catenin
Cell Proliferation
Ovarian Neoplasms
Mice, Inbred BALB C
biology
Cell growth
Plant Extracts
GSK-3β
β-catenin
medicine.disease
Xenograft Model Antitumor Assays
030104 developmental biology
ovarian cancer
Oncology
030220 oncology & carcinogenesis
Immunology
Cancer research
biology.protein
Female
Pyracantha
Ovarian cancer
Research Paper
Subjects
Details
- ISSN :
- 19492553
- Volume :
- 7
- Issue :
- 19
- Database :
- OpenAIRE
- Journal :
- Oncotarget
- Accession number :
- edsair.doi.dedup.....229555c062e4a920f96bddad91bd5b7d