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Regression of mature lymphatic vessels in the cornea by photodynamic therapy
- Source :
- British Journal of Ophthalmology. 98:391-395
- Publication Year :
- 2014
- Publisher :
- BMJ, 2014.
-
Abstract
- Background Corneal (lymph) angiogenesis is a predominant risk-factor for immune rejection after transplantation. Techniques to regress pre-existing pathological corneal lymphatic vessels prior to transplantation are missing so far. Therefore we analysed the possibility to regress corneal lymphatic vessels by photodynamic therapy (PDT), after intrastromal verteporfin injection. Methods Combined hemangiogenesis and lymphangiogenesis was induced in female BALB/c mice using the murine model of suture-induced inflammatory neovascularisation. Thereafter, the treatment group received an intrastromal injection of verteporfin (controls: phosphate buffered saline (PBS)) followed by PDT. Corneas were excised at different time points (1 day, 5 days and 10 days) after PDT and corneal whole mounts were stained with CD31 and LYVE-1 to quantify hemangiogenesis and lymphangiogenesis. Results Whereas blood vessels showed no significant reduction after PDT, lymphatic vessels could significantly be reduced with PDT after intrastromal verteporfin injection: 1 day after PDT, lymphatic vessels were reduced by 62% (p=0.20). After 5 days and 10 days, lymphatic vessels were reduced by 51% and 48% (p Conclusions This study for the first time shows that PDT after corneal intrastromal verteporfin injection can selectively regress lymphatic vessels. This may become a new ‘preconditioning strategy’ to reduce pre-existing corneal lymphatic vessels prior to transplantation and thereby reduce allograft rejection in high-risk patients.
- Subjects :
- Pathology
medicine.medical_specialty
Porphyrins
genetic structures
Angiogenesis
Corneal Stroma
medicine.medical_treatment
Photodynamic therapy
Mice
Cellular and Molecular Neuroscience
Cornea
medicine
Animals
Corneal Neovascularization
Lymphangiogenesis
Glycoproteins
Lymphatic Vessels
Mice, Inbred BALB C
Photosensitizing Agents
business.industry
Membrane Transport Proteins
Verteporfin
eye diseases
Sensory Systems
Surgery
Transplantation
Disease Models, Animal
Ophthalmology
Lymphatic system
medicine.anatomical_structure
Microscopy, Fluorescence
Photochemotherapy
Leukocyte Common Antigens
Female
Lymph
business
medicine.drug
Subjects
Details
- ISSN :
- 14682079 and 00071161
- Volume :
- 98
- Database :
- OpenAIRE
- Journal :
- British Journal of Ophthalmology
- Accession number :
- edsair.doi.dedup.....2255aa147c09a3221e941cc3f8deff29