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Profiling and Identification of Novel Immunogenic Proteins of Staphylococcus hyicus ZC-4 by Immunoproteomic Assay
- Source :
- PLoS ONE, PLoS ONE, Vol 11, Iss 12, p e0167686 (2016)
- Publication Year :
- 2016
- Publisher :
- Public Library of Science, 2016.
-
Abstract
- Staphylococcus hyicus has caused great losses in the swine industry by inducing piglet exudative epidermitis (EE), sow mastitis, metritis, and other diseases and is a threat to human health. The pathogenesis of EE, sow mastitis, and metritis involves the interaction between the host and virulent protein factors of S. hyicus, however, the proteins that interact with the host, especially the host immune system, are unclear. In the present study, immunoproteomics was used to screen the immunogenic proteins of S. hyicus strain ZC-4. The cellular and secreted proteins of S. hyicus strain ZC-4 were obtained, separated by 2D gel electrophoresis, and further analyzed by western blot with S. hyicus strain ZC-4-infected swine serum. Finally, 28 specific immunogenic proteins including 15 cellular proteins and 13 secreted proteins, 26 of which were novel immunogenic proteins from S. hyicus, were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. To further verify their immunogenicity, two representative proteins (acetate kinase [cellular] and enolase [secreted]) were chosen for expression, and the resultant recombinant proteins could react with S. hyicus ZC-4-infected swine serum. In mice, both acetate kinase and enolase activated the immune response by increasing G-CSF and MCP-5 expression, and acetate kinase further activated the immune response by increasing IL-12 expression. Enolase can confer better protection against S.hycius than acetate kinase in mice. For the first time to our knowledge, our results provide detailed descriptions of the cellular and secreted proteins of S. hyicus strain ZC-4. These immunogenic proteins may contribute to investigation and elucidation of the pathogenesis of S. hyicus and provide new candidates for subunit vaccines in the future.
- Subjects :
- 0301 basic medicine
Proteome
Physiology
Swine
lcsh:Medicine
Pathogenesis
Pathology and Laboratory Medicine
Biochemistry
Immunoproteomics
Mice
Immune Physiology
Medicine and Health Sciences
Enzyme-Linked Immunoassays
lcsh:Science
Staphylococcus hyicus
Mammals
Mice, Inbred BALB C
Multidisciplinary
Immune System Proteins
medicine.diagnostic_test
biology
Chemistry
Immunogenicity
Agriculture
Blood proteins
Bacterial Pathogens
Medical Microbiology
Vertebrates
Pathogens
Research Article
Livestock
030106 microbiology
Immunology
Research and Analysis Methods
Microbiology
Antibodies
03 medical and health sciences
Western blot
Antigen
Bacterial Proteins
medicine
Animals
Antigens
Immunoassays
Microbial Pathogens
lcsh:R
Organisms
Biology and Life Sciences
Proteins
biology.organism_classification
Chaperone Proteins
030104 developmental biology
Secretory protein
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Amniotes
Immunologic Techniques
lcsh:Q
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....223eacaeb3eb934c62916b033b01b6f7