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Colostrum IgA1 antibodies recognize antigens from Helicobacter pylori and prevent cytoskeletal changes in human epithelial cells
- Source :
- European Journal of Immunology. 51:2641-2650
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Helicobacter pylori is a Gram-negative bacterium found on the luminal surface of the gastric mucosa in at least 50% of the world's human population. The protective effect of breastfeeding against H. pylori infection has been extensively reported; however, the mechanisms behind this protection remain poorly understood. Human IgA from colostrum has reactivity against H. pylori antigens. Despite that IgA1 and IgA2 display structural and functional differences, their reactivity against H. pylori had not been previously determined. We attested titers and reactivity of human colostrum-IgA subclasses by ELISA, immunoblot, and flow cytometry. Colostrum samples from healthy mothers had higher titers of IgA; and IgA1 mostly recognized H. pylori antigens. Moreover, we found a correlation between IgA1 reactivity and their neutralizing effect determined by inhibition of cytoskeletal changes in AGS cells infected with H. pylori. In conclusion, colostrum-IgA reduces H. pylori infection of epithelial gastric cells, suggesting an important role in preventing the bacteria establishment during the first months of life. As a whole, these results suggest that IgA1 from human colostrum provides protection that may help in the development of the mucosal immune system of newborn children.
- Subjects :
- Immunoglobulin A
Immunology
Population
Helicobacter Infections
fluids and secretions
Immune system
Antigen
Pregnancy
Gastric mucosa
medicine
Humans
Immunology and Allergy
education
Cytoskeleton
Antigens, Bacterial
education.field_of_study
Helicobacter pylori
biology
Colostrum
Epithelial Cells
biology.organism_classification
Antibodies, Bacterial
medicine.anatomical_structure
Gastric Mucosa
Immunoglobulin A, Secretory
biology.protein
Female
Antibody
Subjects
Details
- ISSN :
- 15214141 and 00142980
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi.dedup.....2234e607524659494666006f7d5beff5