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Surface-Modified Mesh Filter for Direct Nucleic Acid Extraction and its Application to Gene Expression Analysis
- Source :
- Advanced healthcare materials. 6(20)
- Publication Year :
- 2017
-
Abstract
- Rapid and convenient isolation of nucleic acids (NAs) from cell lysate plays a key role for onsite gene expression analysis. Here, this study achieves one-step and efficient capture of NA directly from cell lysate by developing a cationic surface-modified mesh filter (SMF). By depositing cationic polymer via vapor-phase deposition process, strong charge interaction is introduced on the surface of the SMF to capture the negatively charged NAs. The NA capturing capability of SMF is confirmed by X-ray photoelectron spectroscopy, fluorescent microscopy, and zeta potential measurement. In addition, the genomic DNAs of Escherichia Coli O157:H7 can be extracted by the SMF from artificially infected food, and fluorescent signal is observed on the surface of SMF after amplification of target gene. The proposed SMF is able to provide a more simplified, convenient, and fast extraction method and can be applied to the fields of food safety testing, clinical diagnosis, or environmental pollutant monitoring.
- Subjects :
- DNA, Bacterial
Lysis
Polymers
Surface Properties
Biomedical Engineering
Analytical chemistry
Pharmaceutical Science
02 engineering and technology
010402 general chemistry
Escherichia coli O157
Spectrum Analysis, Raman
01 natural sciences
Biomaterials
Limit of Detection
Fluorescence microscope
Zeta potential
Solid phase extraction
Detection limit
Chemistry
Photoelectron Spectroscopy
Solid Phase Extraction
021001 nanoscience & nanotechnology
Fluorescence
0104 chemical sciences
Nucleic acid
Biophysics
Microscopy, Electron, Scanning
0210 nano-technology
Biosensor
Genome, Bacterial
Environmental Monitoring
Subjects
Details
- ISSN :
- 21922659
- Volume :
- 6
- Issue :
- 20
- Database :
- OpenAIRE
- Journal :
- Advanced healthcare materials
- Accession number :
- edsair.doi.dedup.....220b49b333deb5aa2b6575e0b1fc8958