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Genome-wide identification and characterization of the ALOG gene family in Petunia
- Source :
- BMC Plant Biology, BMC Plant Biology, Vol 19, Iss 1, Pp 1-15 (2019)
- Publication Year :
- 2019
- Publisher :
- BioMed Central, 2019.
-
Abstract
- Background The ALOG (Arabidopsis LSH1 and Oryza G1) family of proteins, namely DUF640 (domain of unknown function 640) domain proteins, were found in land plants. Functional characterization of a few ALOG members in model plants such as Arabidopsis and rice suggested they play important regulatory roles in plant development. The information about its evolution, however, is largely limited, and there was no any report on the ALOG genes in Petunia, an important ornamental species. Results The ALOG genes were identified in four species of Petunia including P. axillaris, P. inflata, P. integrifolia, and P. exserta based on the genome and/or transcriptome databases, which were further confirmed by cloning from P. hybrida ‘W115’ (Mitchel diploid), a popular laboratorial petunia line susceptible to genetic transformation. Phylogenetic analysis indicated that Petunia ALOG genes (named as LSHs according to their closest Arabidopsis homologs) were grouped into four clades, which can be further divided into eight groups, and similar exon-intron structure and motifs are reflected in the same group. The PhLSH genes of hybrid petunia ‘W115’ were mainly derived from P. axillaris. The qPCR analysis revealed distinct spatial expression patterns among them suggesting potentially functional diversification. Moreover, over-expressing PhLSH7a and PhLSH7b in Arabidopsis uncovered their functions in the development of both vegetative and reproductive organs. Conclusions Petunia genome includes 11 ALOG genes that can be divided into eight distinct groups, and they also show different expression patterns. Among these genes, PhLSH7b and PhLSH7a play significant roles in plant growth and development, especially in fruit development. Our results provide new insight into the evolution of ALOG gene family and have laid a good foundation for the study of petunia LSH gene in the future.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Plant Science
Biology
01 natural sciences
Genome
Petunia
03 medical and health sciences
Species Specificity
Gene Expression Regulation, Plant
lcsh:Botany
Arabidopsis
ALOG genes
Plant development
Gene family
Expression pattern
Amino Acid Sequence
Gene
Phylogeny
Plant Proteins
Cloning
Genetics
Phylogenetic tree
Gene Expression Profiling
biology.organism_classification
lcsh:QK1-989
030104 developmental biology
Multigene Family
DUF640 domain
Domain of unknown function
Transcription factor
Transcriptome
Sequence Alignment
Genome, Plant
010606 plant biology & botany
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14712229
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- BMC Plant Biology
- Accession number :
- edsair.doi.dedup.....2209b709aaeec205942d56e681d4701e