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Circulating MicroRNA-4739 May Be a Potential Biomarker of Critical Limb Ischemia in Patients with Diabetes
- Source :
- BioMed Research International, Vol 2018 (2018), BioMed Research International
- Publication Year :
- 2018
- Publisher :
- Hindawi Limited, 2018.
-
Abstract
- Critical limb ischemia (CLI) is the most severe manifestation of peripheral artery disease, which is common but rarely diagnosed. Noninvasive biomarkers are urgently required to assist in the diagnosis of CLI. Accumulating evidence indicates that miRNAs play an important role in the development of various diseases. In this study, microarray profiling revealed 11 miRNAs with significantly altered expression in four T2DM patients with CLI compared with that in four sex- and age-matched T2DM patients without CLI. In independent cohorts, qRT-PCR validation confirmed the increased miRNA-4739 level in patients with CLI versus patients without CLI. miRNA-4739 levels increased with FPG and HbA1c (all P < 0.05). After adjusting for the risk factors, miRNA-4739 levels were found to be associated with an increased odds ratio (OR) of T2DM with CLI (OR =12.818, 95% confidence intervals (CI) 1.148 to 143.143, P = 0.038). ROC curve analysis revealed that the area under the curve (AUC) of miR-4739+confounding risk factors was 0.94 (95% CI 0.891 to 0.998, P < 0.001), which was higher than that of confounding risk factors (AUC 0.94 vs. 0.91, 95% CI -0.122 to 0.060, P > 0.05) and of miR-4739 (AUC 0.94 vs. 0.69, 95% CI -0.399 to -0.101, P < 0.001), respectively. We conclude that elevated plasma miRNA-4739 levels are independently associated with CLI in T2DM patients. miRNA-4739 is implicated as a novel diagnostic marker and a potential therapeutic target for CLI in diabetes.
- Subjects :
- 0301 basic medicine
Oncology
Male
medicine.medical_specialty
Article Subject
lcsh:Medicine
Disease
Real-Time Polymerase Chain Reaction
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
Ischemia
Risk Factors
Diabetes mellitus
Internal medicine
microRNA
medicine
Humans
Circulating MicroRNA
RNA, Messenger
Aged
General Immunology and Microbiology
business.industry
Foot
Gene Expression Profiling
lcsh:R
Case-control study
Reproducibility of Results
General Medicine
Critical limb ischemia
Middle Aged
medicine.disease
Diabetic Foot
Gene expression profiling
body regions
MicroRNAs
030104 developmental biology
Real-time polymerase chain reaction
Logistic Models
Diabetes Mellitus, Type 2
ROC Curve
Case-Control Studies
Female
medicine.symptom
business
Biomarkers
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 23146141 and 23146133
- Volume :
- 2018
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....22014ec98c88d423d9a05c927d663b59