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The inflammation and reactive oxygen species regulated by Nrf2 and NF-κB signaling pathways in 630-nm light-emitting diode irradiation treated THP-1 monocytes/macrophages
- Source :
- Lasers in Medical Science. 36:1411-1419
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Because of a large number of macrophages and its secreted pro-inflammatory factors in the synovial fluid of patients with rheumatoid arthritis, the present study aimed to investigate the effect and mechanism of 630-nm LED exposure on monocytes/macrophages and its anti-inflammatory effect. The THP-1 monocytes and PMA-induced THP-1 macrophages (THP-1 macrophages) were employed and irradiated by 630-nm LED for different time and times, and then measure the pro-inflammatory cytokines production by RT-qPCR and Milliplex MAP Multiplex assay, the proteins involved in inflammation pathway and reactive oxygen species (ROS) levels in the cells were detected by Western blot and DCFH-DA method. The exposure dose of red LED (15.3 J/cm2, 30.6 J/cm2) were determined as no-influence on the cell proliferation, the pro-inflammatory factors TNF-α and IL-1β mRNAs, and secretions in supernatant of THP-1 macrophages were significantly decreased after LED exposure. The ROS production was blocked in THP-1 monocytes and THP-1 macrophages after treatment of LED. Finally, the phosphorylated NF-κB proteins which involved in inflammation pathway significantly decreased, and its inhibitors Nrf2 were slightly upregulated. The effects of LED anti-inflammation response are dependent on the mechanism of inhibiting ROS level and regulating NF-κB signaling pathways by increasing Nrf2 expression in the cells. It is suggested that 630-nm LED could decrease pro-inflammation in immune cells, and it may be a beneficial adjunct therapy in relieving inflammation of patients with rheumatoid arthritis.
- Subjects :
- Lipopolysaccharides
NF-E2-Related Factor 2
Inflammation
Dermatology
Monocytes
Arthritis, Rheumatoid
030207 dermatology & venereal diseases
03 medical and health sciences
0302 clinical medicine
Immune system
Downregulation and upregulation
Western blot
medicine
Humans
THP1 cell line
chemistry.chemical_classification
Reactive oxygen species
medicine.diagnostic_test
Cell growth
Macrophages
NF-kappa B
030206 dentistry
Molecular biology
chemistry
Cytokines
Surgery
medicine.symptom
Signal transduction
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 1435604X and 02688921
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Lasers in Medical Science
- Accession number :
- edsair.doi.dedup.....220044715874954d570cfa01682ac4a1