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Protein and gene integration analysis through proteome and transcriptome brings new insight into salt stress tolerance in pigeonpea (Cajanus cajan L.)
- Source :
- International journal of biological macromolecules. 164
- Publication Year :
- 2020
-
Abstract
- Salt stress is a major constrain to the productivity of nutritionally rich pigeonpea, an important legume of SE Asia and other parts of the world. The present study provides a comprehensive insight on integrated proteomic and transcriptomic analysis of root and shoot tissues of contrasting pigeonpea varieties (ICP1071- salt-sensitive; ICP7- salt-tolerant) to unravel salt stress induced pathways. Proteome analysis revealed 82 differentially expressed proteins (DEPs) with ≥±1.5 fold expression on 2-Dimensional (2D) gel. Of these, 25 DEPs identified through MALDI-TOF/TOF were classified using Uniprot software into functional categories. Pathways analyses using KAAS server showed the highest abundance of functional genes regulating metabolisms of carbohydrate followed by protein folding/degradation, amino acids and lipids. Expression studies on six genes (triosephosphate isomerase, oxygen evolving enhancer protein 1, phosphoribulokinase, cysteine synthase, oxygen evolving enhancer protein 2 and early nodulin like protein 2) with ≥±3 fold change were performed, and five of these showed consistency in transcript and protein expressions. Transcript analysis of root and shoot led to positive identification of 25 differentially expressed salt-responsive genes, with seven genes having ≥±5 fold change have diverse biological functions. Our combinatorial analysis suggests important role of these genes/proteins in providing salt tolerance in pigeonpea.
- Subjects :
- Proteomics
Proteome
02 engineering and technology
Cysteine synthase
Biochemistry
Salt Stress
Triosephosphate isomerase
Transcriptome
03 medical and health sciences
Cajanus
Structural Biology
Gene Expression Regulation, Plant
Enhancer
Molecular Biology
Gene
030304 developmental biology
Plant Proteins
0303 health sciences
biology
General Medicine
Salt Tolerance
021001 nanoscience & nanotechnology
biology.organism_classification
Fold change
biology.protein
0210 nano-technology
Subjects
Details
- ISSN :
- 18790003
- Volume :
- 164
- Database :
- OpenAIRE
- Journal :
- International journal of biological macromolecules
- Accession number :
- edsair.doi.dedup.....21fe36d80584ddaa28a5e0ffa0780c22